Bifunctional P-450/nadph-P450 Reductase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
PHE-165
TYR-166
14.7
14.5
12.9
-20.9
73.9
74.8
-8.8
TYR-166
ARG-167
15.7
15.5
-8.5
2.9
138.0
131.6
-70.0
ARG-167
ASP-168
13.0
12.9
13.4
17.9
38.6
27.8
152.5
ASP-168
GLN-169
12.7
11.8
-53.6
19.3
104.1
88.0
-57.8
GLN-169
PRO-170
9.0
8.5
-23.6
15.6
28.8
18.0
30.1
PRO-170
HIS-171
6.4
6.3
-12.9
16.4
51.4
55.7
33.3
HIS-171
PRO-172
6.4
7.1
-3.6
-1.2
105.0
102.8
-35.0
PRO-172
PHE-173
5.8
6.7
7.0
-11.4
120.3
120.7
26.0
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-230
ASP-231
8.8
8.9
-35.4
66.8
109.3
92.3
-29.6
ASP-231
ASP-232
8.3
8.9
-28.1
16.8
33.0
35.5
49.5
ASP-232
LEU-233
7.6
7.0
-2.7
-11.0
82.9
94.7
-33.4
LEU-233
LEU-234
6.0
5.3
8.2
-0.9
74.8
73.0
21.7
LEU-234
THR-235
2.3
1.5
-2.9
-3.9
168.5
170.8
-30.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
HIS-236
MET-237
4.5
4.3
-6.6
-9.6
71.0
85.7
14.9
MET-237
LEU-238
4.1
4.0
7.9
15.5
53.7
56.7
16.2
LEU-238
ASN-239
2.6
2.3
-7.0
20.7
171.7
166.4
81.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ILE-259
THR-260
4.5
4.9
0.6
-6.8
78.1
74.6
-14.1
THR-260
PHE-261
7.4
7.7
-1.4
15.2
87.2
92.7
24.5
PHE-261
LEU-262
6.5
6.8
-2.5
-2.1
131.2
142.7
-32.8
LEU-262
ILE-263
4.1
4.4
-2.1
26.3
149.0
146.0
114.4
ILE-263
ALA-264
6.0
6.6
6.7
-6.2
96.1
89.3
-27.8
ALA-264
GLY-265
9.5
9.6
-6.1
11.9
75.5
102.6
6.1
GLY-265
HIS-266
9.4
8.7
-41.3
58.3
132.8
167.4
140.1
HIS-266
GLU-267
9.6
7.4
-0.6
-32.2
148.7
124.5
-175.2
GLU-267
THR-268
10.1
10.0
18.7
-36.3
82.9
96.4
-82.4
THR-268
THR-269
13.8
13.4
-32.7
8.3
81.6
90.1
-38.2
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees