Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ALA-110   LEU-111  20.2 20.2 14.8 -14.8 102.5 105.6 8.6
 LEU-111   ASP-112  17.0 16.9 3.4 -16.8 59.8 59.6 -8.9
 ASP-112   THR-113  14.0 14.0 -2.4 -13.6 60.6 67.3 -12.5
 THR-113   LYS-114  10.7 10.7 -5.8 14.6 112.0 121.2 7.8
 LYS-114   GLY-115  7.8 8.5 16.8 7.5 70.9 77.9 20.8
 GLY-115   PRO-116  5.2 5.6 -8.3 17.7 94.3 82.4 -2.7
 PRO-116   GLU-117  1.6 1.9 86.1 -38.9 36.1 45.3 92.1
 GLU-117   ILE-118  2.5 2.6 -18.9 -1.4 78.7 82.5 -5.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ALA-214   VAL-215  9.8 11.0 25.7 1.4 34.6 34.5 83.0
 VAL-215   ASP-216  7.2 8.3 -14.4 22.7 131.4 153.7 6.7
 ASP-216   LEU-217  7.0 8.8 -72.6 45.7 124.0 123.1 -8.7
 LEU-217   PRO-218  3.7 5.2 16.2 -7.8 64.5 66.5 20.5
 PRO-218   ALA-219  4.0 5.2 9.5 7.4 119.7 124.2 -10.6
 ALA-219   VAL-220  5.9 7.0 -17.5 10.2 117.7 97.0 -22.1
 VAL-220   SER-221  5.4 5.7 46.9 -34.4 59.3 65.5 2.4
 SER-221   GLU-222  3.1 3.1 13.7 5.3 24.2 27.6 43.7
 GLU-222   LYS-223  4.1 4.0 3.8 -10.2 108.3 101.7 6.7
 LYS-223   ASP-224  7.0 7.3 5.8 -4.7 105.8 102.5 15.1
 ASP-224   ILE-225  7.5 7.8 -9.9 15.8 136.3 141.3 15.2
 ILE-225   GLN-226  8.8 8.5 -5.7 -8.9 105.3 109.8 -1.5
 GLN-226   ASP-227  10.3 10.4 20.8 -17.1 127.1 126.4 -24.3
 ASP-227   LEU-228  12.2 12.5 11.1 -6.1 82.4 88.5 9.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees