T7 RNA Polymerase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-43
TYR-44
12.8
12.9
-42.7
62.7
12.6
1.2
-13.0
TYR-44
GLU-45
14.1
14.3
-5.7
84.5
75.6
64.8
-19.0
GLU-45
MET-46
14.8
13.7
-176.3
19.4
144.9
90.9
-42.5
MET-46
GLY-47
14.5
10.1
-158.7
-9.1
96.1
43.5
140.5
GLY-47
GLU-48
17.9
9.5
172.1
2.3
98.0
154.0
-79.6
GLU-48
ALA-49
20.1
10.9
-12.9
12.3
150.8
86.2
0.6
ALA-49
ARG-50
20.7
9.4
0.6
-6.7
68.2
106.5
3.6
ARG-50
PHE-51
17.1
5.7
17.6
-5.8
120.2
157.5
-5.3
PHE-51
ARG-52
16.6
6.4
5.5
7.8
85.0
128.3
-3.0
ARG-52
LYS-53
20.1
7.8
-14.8
4.7
154.7
91.9
-4.3
LYS-53
MET-54
19.8
6.0
-34.7
28.5
98.7
57.9
2.9
MET-54
PHE-55
17.4
2.5
-48.5
40.8
70.7
8.8
8.2
PRO-72
LEU-73
12.1
13.0
-161.5
-34.3
8.1
18.1
149.4
LEU-73
ILE-74
10.6
11.3
-5.6
11.5
57.0
60.0
-6.9
ILE-74
THR-75
10.4
9.2
-22.5
-1.7
73.3
69.2
6.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
THR-256
ARG-257
13.5
15.1
-29.5
33.7
72.4
56.2
-4.7
ARG-257
ALA-258
12.6
13.4
163.4
27.4
127.0
145.6
129.2
ALA-258
GLY-259
11.0
10.9
-141.0
-154.1
113.6
45.2
-11.9
GLY-259
ALA-260
8.9
13.9
-80.2
-3.8
173.3
55.3
-40.2
ALA-260
LEU-261
9.0
15.6
-15.5
43.2
114.3
53.9
-12.9
LEU-261
ALA-262
6.0
12.7
-45.5
-58.1
65.6
30.2
71.7
ALA-262
GLY-263
8.5
11.6
77.7
-19.8
71.7
132.7
-13.1
GLY-263
ILE-264
9.6
14.9
-28.6
0.9
129.9
64.6
-6.6
ILE-264
SER-265
6.6
15.1
-64.7
-34.2
56.1
64.5
73.4
SER-265
PRO-266
6.4
11.7
-101.9
-13.7
121.8
29.5
-155.4
PRO-266
MET-267
6.5
12.2
-7.7
-38.8
111.8
53.4
-14.5
MET-267
PHE-268
9.6
9.5
119.8
61.6
131.6
151.2
110.2
PHE-268
GLN-269
11.7
11.8
19.7
-8.9
154.1
150.6
-9.0
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees