T7 DNA Polymerase
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
HIS-498
GLU-499
10.6
11.1
-15.8
-1.5
45.2
50.0
28.6
GLU-499
ILE-500
12.0
13.3
3.9
2.4
100.0
119.7
-12.5
ILE-500
LEU-501
9.6
10.9
42.1
-52.0
46.5
62.0
-29.1
LEU-501
ASN-502
10.7
12.4
64.8
-42.9
96.6
86.3
18.2
ASN-502
GLY-503
14.4
16.0
110.0
-16.3
137.4
140.8
-242.6
GLY-503
ASP-504
14.2
17.3
-4.3
-112.6
31.3
57.9
421.3
ASP-504
ILE-505
14.9
15.4
80.4
-2.2
108.2
126.1
-91.3
ILE-505
HIS-506
11.9
12.8
-15.0
3.7
11.3
10.0
31.7
HIS-506
THR-507
12.6
13.1
-11.7
7.2
76.8
71.8
14.4
THR-507
LYS-508
16.1
16.8
-2.3
5.2
98.8
91.2
-2.0
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
THR-559
PRO-560
14.7
13.5
8.5
-20.9
95.9
67.2
7.1
PRO-560
ALA-561
16.3
15.3
26.3
-8.9
110.8
115.4
-14.2
ALA-561
ILE-562
13.5
13.5
18.6
9.9
48.3
58.0
64.0
ILE-562
ALA-563
11.2
10.8
9.5
-8.9
56.7
63.8
-3.0
ALA-563
ALA-564
14.2
13.6
2.1
3.5
121.3
128.7
-10.7
ALA-564
LEU-565
15.4
14.8
-4.3
0.8
86.1
83.4
-6.3
LEU-565
ARG-566
12.4
11.6
-2.2
7.2
145.6
143.2
13.9
ARG-566
GLU-567
12.4
11.8
8.6
-5.4
83.1
90.7
-7.0
GLU-567
SER-568
16.2
15.5
-7.3
-4.2
52.9
56.3
12.4
SER-568
ILE-569
16.3
15.5
3.2
11.1
70.6
70.3
4.9
ILE-569
GLN-570
14.5
13.6
3.5
-4.3
37.3
46.3
-6.0
GLN-570
GLN-571
16.3
16.0
-3.3
10.7
78.1
68.7
-13.3
GLN-571
THR-572
19.8
19.1
-15.5
5.3
58.7
62.1
30.4
THR-572
LEU-573
19.5
18.3
0.6
1.7
50.3
51.8
-8.6
LEU-573
VAL-574
19.0
18.1
-5.9
7.8
148.2
137.0
-1.7
VAL-574
GLU-575
20.3
20.0
5.5
-4.7
89.5
101.8
-3.0
GLU-575
SER-576
23.9
23.7
12.1
-14.4
35.7
42.4
-8.1
SER-576
SER-577
23.2
23.6
-6.9
5.0
124.8
136.8
-2.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees