Monoclonal Antibody 2D12.5, Lambda Light Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-113   SER-114  8.6 8.6 27.6 -38.5 24.0 21.7 -90.8
 SER-114   SER-115  8.3 8.2 -53.0 40.9 128.4 126.4 74.6
 SER-115   PRO-116  8.6 8.5 5.6 10.8 64.4 46.4 275.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-136   CYS-137  5.7 6.0 29.2 -24.5 73.9 58.5 94.8
 CYS-137   THR-138  5.6 5.1 5.0 -16.5 39.3 45.0 -118.4
 THR-138   ILE-139  2.5 2.1 34.8 -30.5 39.8 45.6 133.2
 ILE-139   THR-140  3.3 2.0 36.4 -20.9 53.5 50.1 115.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 THR-148   VAL-149  11.3 11.2 -19.0 9.6 165.7 162.6 -55.6
 VAL-149   ASP-150  11.2 11.2 66.0 -57.6 50.7 65.0 69.3
 ASP-150   TRP-151  14.3 14.2 -31.6 47.6 170.6 166.9 153.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-173   LYS-174  7.3 6.5 -6.6 11.0 74.2 74.3 106.8
 LYS-174   TYR-175  4.7 4.2 36.0 -27.8 123.7 118.3 -160.5
 TYR-175   MET-176  2.5 1.9 -16.8 -9.3 16.4 10.3 232.9
 MET-176   ALA-177  2.3 2.3 -14.4 -7.4 80.9 79.9 -51.8
 ALA-177   SER-178  3.9 4.3 17.8 -10.9 173.4 166.5 -102.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees