Fab Fragment From Human Immunoglobulin Igg1 (Lambda, Hil)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-120   SER-121  4.9 5.2 -3.4 -14.1 160.3 162.3 -110.0  SER-121   ALA-122  5.9 6.2 27.9 19.2 55.6 64.3 123.5  ALA-122   SER-123  3.0 3.4 9.1 -11.9 76.6 41.8 -23.7  SER-123   THR-124  0.5 1.8 -134.4 78.0 68.8 70.8 187.2  THR-124   LYS-125  3.7 3.6 -25.9 16.1 85.6 89.7 2.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees