Periplasmic Oligopeptide-Binding Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-245   ASN-246  10.9 11.2 9.9 -16.9 85.1 81.5 2.3  ASN-246   ASN-247  7.2 7.4 22.1 -18.5 72.2 87.1 75.2  ASN-247   MET-248  6.8 7.5 -5.5 0.9 168.8 171.7 -31.7  MET-248   PRO-249  5.4 6.2 12.6 -9.9 60.3 55.7 24.3  PRO-249   ILE-250  6.7 7.9 6.0 -18.8 69.0 62.2 3.0  ILE-250   GLU-251  5.3 7.4 18.0 4.0 16.6 10.2 85.1  GLU-251   LEU-252  5.5 8.2 8.8 -53.8 76.8 69.6 -61.9  LEU-252   PHE-253  4.8 5.9 28.8 -10.8 105.4 114.6 -69.1  PHE-253   GLN-254  2.1 2.9 2.3 -0.1 53.9 57.5 -26.4  GLN-254   LYS-255  0.8 0.6 -3.4 1.5 135.7 128.1 6.7  LYS-255   LEU-256  2.4 2.3 11.0 -4.8 117.0 117.2 0.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-268   TYR-269  5.7 5.5 -6.0 -4.5 59.0 61.2 -0.2  TYR-269   LEU-270  2.6 2.2 -11.3 -9.9 42.3 48.9 61.2  LEU-270   CYS-271  1.4 1.3 6.6 1.2 85.4 91.0 30.5  CYS-271   THR-272  3.0 3.1 2.7 -6.5 69.1 68.6 -3.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-485   VAL-486  3.2 3.3 8.6 -13.0 99.8 96.3 -3.2  VAL-486   ASN-487  2.5 1.6 3.6 1.3 94.0 94.1 32.9  ASN-487   ALA-488  3.2 3.1 -11.8 3.1 28.6 24.0 45.8  ALA-488   ARG-489  4.1 3.7 1.0 -8.0 104.0 100.9 -43.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees