Helicase Pcra

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 VAL-381   GLY-382  3.8 3.3 -26.2 20.6 41.6 50.3 1.8
 GLY-382   GLY-383  5.4 4.9 -62.5 29.1 57.2 55.1 13.3
 GLY-383   LEU-384  2.9 1.9 145.1 54.7 114.8 91.5 13.7
 LEU-384   LYS-385  3.6 1.9 94.7 27.4 105.9 65.8 74.3
 LYS-385   PHE-386  6.7 5.3 22.7 10.4 130.5 126.9 -3.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-538   ALA-539  16.9 18.7 73.8 -38.3 91.9 94.0 2.9
 ALA-539   LEU-540  15.4 19.0 7.1 -10.4 121.9 152.1 -0.2
 LEU-540   ILE-541  17.3 19.5 -9.3 21.5 24.6 58.2 -4.9
 ILE-541   SER-542  16.0 16.8 -46.6 -102.9 105.5 121.5 159.5
 ASP-543   LEU-544  11.7 11.4 89.6 -15.7 78.8 148.8 -41.5
 LEU-544   ASP-545  12.7 10.3 65.8 -22.0 144.5 69.9 -32.0
 ASP-545   GLU-546  14.2 7.6 166.0 24.8 100.2 102.2 54.4
 GLU-546   LEU-547  16.3 4.4 158.7 -32.5 40.5 35.0 -152.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees