Crispr-Associated Endonuclease Cas9/csn1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-711   GLN-712  15.8 15.3 12.2 -30.4 97.6 78.1 3.7  GLN-712   VAL-713  13.2 12.9 161.3 3.4 91.8 69.9 98.9  ASP-718   SER-719  10.7 6.1 -31.1 -9.1 95.0 137.9 -40.1  SER-719   LEU-720  7.7 7.9 -152.1 -5.3 105.5 111.6 -34.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees