DNA Topoisomerase I

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASN-623   ARG-624  8.1 8.6 -0.6 2.1 86.5 85.0 -26.3
 ARG-624   ALA-625  4.3 4.8 5.1 -1.4 152.7 155.5 -19.4
 ALA-625   VAL-626  4.6 4.4 -1.8 -4.4 42.6 52.4 30.9
 VAL-626   ALA-627  5.8 6.4 -14.4 24.0 87.8 102.5 28.6
 ALA-627   ILE-628  4.2 6.0 -69.3 42.4 72.1 68.2 52.8
 ILE-628   LEU-629  0.9 3.0 -5.5 13.1 20.4 13.8 -57.6
 LEU-629   CYS-630  2.1 3.6 -1.4 2.1 88.2 78.4 32.6
 CYS-630   ASN-631  5.7 7.3 -1.7 12.8 126.8 112.4 38.7
 ASN-631   HIS-632  5.0 7.4 -144.1 -2.3 139.6 137.0 -906.4
 HIS-632   GLN-633  6.2 9.0 -93.6 80.9 106.6 141.1 -243.9
 MET-644   MET-645  2.6 2.8 153.1 20.9 61.8 37.6 432.8
 MET-645   ASN-646  4.0 4.5 -43.3 -3.9 136.9 130.4 -221.4
 ASN-646   LEU-647  1.4 1.4 -18.7 -1.2 77.1 77.0 42.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLN-704   ALA-705  7.1 7.0 9.0 -6.0 86.1 86.5 17.5
 ALA-705   THR-706  5.0 4.7 16.7 -0.1 25.1 20.1 113.8
 THR-706   ASP-707  6.2 5.8 -10.3 4.1 133.1 123.4 -62.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees