Type II Restriction Enzyme PvuII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-38   LEU-39  3.7 2.9 4.9 12.8 80.5 65.9 12.8
 LEU-39   LEU-40  2.1 2.8 -20.1 8.3 101.1 95.8 -6.3
 LEU-40   GLN-41  1.6 1.5 -12.3 -8.3 38.5 35.6 44.4
 GLN-41   VAL-42  2.8 2.6 -0.9 4.1 91.7 86.5 -2.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-47   LEU-48  10.7 11.6 -5.6 9.5 40.2 40.1 -12.5
 LEU-48   THR-49  9.4 10.6 -1.5 -1.6 47.4 45.2 -15.0
 THR-49   VAL-50  7.4 8.7 8.9 -28.1 104.3 117.6 32.1
 VAL-50   LEU-51  6.2 6.6 -8.2 -10.1 15.8 16.2 42.1
 LEU-51   PRO-52  8.2 7.5 13.4 -32.0 121.6 125.0 64.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees