Fab Antibody Light Chain

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-29   LYS-30  13.0 13.1 27.2 -5.9 10.3 15.7 146.3  LYS-30   THR-31  11.5 11.3 -15.1 3.3 109.0 113.5 -48.0  THR-31   TYR-32  9.4 9.6 -7.1 2.4 127.3 131.2 4.7  TYR-32   LEU-33  6.4 6.7 6.7 -5.7 39.1 41.4 0.5  LEU-33   ILE-34  6.1 6.2 -4.8 6.0 138.7 142.9 23.6  ILE-34   TRP-35  5.7 5.6 1.0 -5.3 35.5 34.8 -22.5  TRP-35   VAL-36  5.0 5.0 -4.5 11.6 117.9 124.5 16.5  VAL-36   PHE-37  3.4 3.3 15.5 13.3 20.1 19.2 180.4  PHE-37   GLN-38  3.3 3.0 139.3 -144.6 72.4 65.8 -58.7  GLN-38   ARG-39  3.9 4.0 -32.7 6.1 163.1 161.4 -149.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-45   ARG-46  8.0 7.4 -0.6 15.4 121.7 121.5 31.6  ARG-46   LEU-47  7.6 7.6 -8.8 -1.9 63.8 59.5 42.5  LEU-47   ILE-48  8.9 8.7 -2.0 -3.9 132.6 130.6 -56.5  ILE-48   PHE-49  9.8 9.7 -8.6 10.2 154.7 156.2 44.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-106   ALA-106A  6.8 6.4 -17.7 16.2 141.0 144.2 -37.0  ALA-106A   ARG-108  9.2 9.0 13.9 -18.3 43.6 42.4 -78.7  ARG-108   ALA-109  7.9 8.0 -8.8 1.4 147.5 144.7 3.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees