4M5.3 Anti-Fluorescein Single Chain Antibody Fragment

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-155   ASP-156  42.0 35.7 12.9 -2.4 152.9 125.3 -21.6
 LEU-155   ASP-156  8.5 5.2 12.9 -2.4 73.9 105.4 3.0
 ASP-156   GLU-157  7.4 7.0 2.5 20.3 142.8 90.6 -10.3
 LEU-155   ASP-156  42.0 35.7 12.9 -2.4 152.9 125.3 -21.6
 LEU-155   ASP-156  8.5 5.2 12.9 -2.4 73.9 105.4 3.0
 ASP-156   GLU-157  7.4 7.0 2.5 20.3 142.8 90.6 -10.3
 GLU-157   THR-158  5.4 8.4 25.1 75.3 80.5 150.1 80.6
 THR-158   GLY-159  8.0 10.2 -19.1 172.3 33.5 89.7 -81.6
 GLY-159   GLY-160  9.3 13.2 -175.0 -19.7 92.0 90.0 8.6
 GLY-160   GLY-161  8.6 16.5 175.9 129.0 78.4 159.7 15.3
 GLY-161   LEU-162  12.0 16.8 126.3 28.9 116.4 109.0 52.8
 LEU-162   VAL-163  14.6 14.2 -167.6 84.9 25.5 121.9 -11.3
 VAL-163   GLN-164  15.5 15.8 154.8 38.4 109.5 113.5 -82.5
 GLN-164   PRO-165  17.8 19.1 172.2 -24.9 143.7 134.3 134.1
 PRO-165   GLY-166  16.7 17.7 -141.7 -7.4 36.0 112.4 -140.8
 GLY-166   GLY-167  15.3 17.5 8.0 -34.8 75.3 78.5 5.8
 GLY-167   ALA-168  14.4 13.8 -3.6 -24.6 88.5 130.3 91.8
 LYS-170   LEU-171  8.4 7.4 -1.1 -3.7 157.9 136.0 -2.5
 LEU-171   SER-172  6.3 5.1 -25.5 20.1 86.6 69.0 4.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 SER-216   VAL-217  10.2 12.0 -176.0 -37.6 84.6 71.3 31.6
 VAL-217   LYS-218  6.6 8.5 155.0 -70.9 147.5 63.6 -3.7
 LYS-218   GLY-219  5.5 5.0 -12.8 171.4 57.1 91.2 -30.2
 GLY-219   ARG-220  4.3 2.8 135.1 -1.8 60.0 83.9 -57.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-246   TYR-247  5.9 11.4 -1.3 44.0 128.1 85.0 -12.3
 TYR-247   TYR-248  2.9 9.8 -5.1 4.3 61.9 92.3 -1.8
 TYR-248   CYS-249  2.0 6.1 12.1 -60.2 46.0 79.7 146.8
 CYS-249   THR-250  4.2 4.2 -24.3 -12.9 65.2 92.7 -107.3
 THR-250   GLY-251  7.7 2.0 19.4 -25.6 60.2 104.2 5.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees