Nickel-Binding Periplasmic Protein

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-197   VAL-198  12.9 12.4 7.9 -0.1 83.0 86.5 7.7  VAL-198   ILE-199  15.1 14.6 5.6 -14.7 158.8 147.7 48.8  ILE-199   PRO-200  14.1 13.4 -6.1 -13.4 93.3 101.7 -4.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-211   GLY-212  4.0 3.8 -14.0 -10.4 48.2 54.0 23.6  GLY-212   ASP-213  5.0 4.9 7.4 7.7 125.1 113.1 -14.8  ASP-213   ILE-214  7.2 7.5 -14.8 -2.5 21.2 6.8 198.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-215   LEU-216  4.0 4.1 11.5 4.7 65.3 58.9 96.2  LEU-216   LEU-217  1.5 1.8 -5.9 8.8 86.4 86.5 56.1  LEU-217   TYR-218  4.3 4.1 20.4 -23.6 46.2 37.9 -62.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-233   GLN-234  11.2 11.1 10.1 -9.4 57.3 61.8 -20.1  GLN-234   ASN-235  13.1 12.9 -11.6 34.3 118.7 119.1 97.4  ASN-235   PRO-236  10.6 10.2 1.5 -6.6 124.6 123.4 54.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  MET-472   MET-473  11.0 10.8 -3.0 3.4 35.7 42.6 19.3  MET-473   VAL-474  9.5 9.2 18.4 -10.2 96.9 96.7 -56.1  VAL-474   VAL-475  6.0 5.7 0.3 2.4 159.5 155.9 17.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-476   LYS-477  2.8 2.5 17.9 -4.0 41.2 51.0 124.6  LYS-477   PRO-478  4.6 5.0 8.7 -5.8 117.9 125.5 -13.7  PRO-478   GLU-479  8.0 8.3 -2.9 9.1 55.2 50.8 -39.2  GLU-479   LEU-480  9.7 9.8 -2.8 -3.9 20.6 26.8 57.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-482   ILE-483  8.6 8.0 -4.9 0.1 59.3 64.7 -22.0  ILE-483   PRO-484  5.2 4.5 -12.3 5.4 36.5 39.5 36.5  PRO-484   TYR-485  5.3 4.6 -1.0 -7.5 89.2 89.6 35.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees