Adp-Ribosylation Factor 1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-20   LEU-21  4.6 5.3 -6.5 4.3 120.8 125.7 -1.7  LEU-21   MET-22  2.9 3.6 21.5 -7.8 121.3 123.2 -33.0  MET-22   VAL-23  6.5 6.9 5.1 9.0 71.9 72.5 15.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-56   GLU-57  14.3 12.1 28.3 -3.8 67.3 62.1 38.3  GLU-57   TYR-58  17.2 13.5 8.4 7.8 31.3 44.6 13.8  TYR-58   LYS-59  17.7 15.0 -6.6 13.2 126.8 126.2 9.1  LYS-59   ASN-60  20.1 17.0 -4.2 -8.4 74.5 73.6 -2.7  ASN-60   ILE-61  20.7 16.0 17.6 -6.5 99.5 79.8 -2.9  ILE-61   SER-62  17.0 12.2 -18.2 28.9 49.8 53.3 -21.0  SER-62   PHE-63  14.5 9.6 -2.2 9.8 102.8 123.0 1.3  PHE-63   THR-64  11.0 8.2 13.7 -45.1 146.8 138.3 55.1  THR-64   VAL-65  8.8 6.4 19.3 35.4 68.6 65.2 81.9  VAL-65   TRP-66  7.2 7.5 7.3 -9.4 134.7 113.8 1.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-82   GLN-83  4.9 4.8 39.9 -2.8 90.7 111.8 -21.9  GLN-83   ASN-84  6.9 8.2 -166.2 -167.5 88.6 70.0 -53.3  ASN-84   THR-85  7.0 7.8 1.4 -15.1 113.6 126.6 22.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees