Bifunctional P-450:nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-174   THR-175  15.5 16.3 -1.0 6.3 102.2 99.4 13.5
 THR-175   SER-176  16.2 16.7 -1.7 -0.5 111.9 107.8 -13.0
 SER-176   MET-177  13.0 13.4 1.3 -6.7 130.4 132.3 22.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ASP-182   GLU-183  11.0 11.0 2.5 4.1 121.4 124.3 -9.4
 GLU-183   ALA-184  10.5 10.3 -2.3 -3.8 110.3 110.3 -4.3
 ALA-184   MET-185  7.5 7.2 -0.1 5.4 144.4 145.5 9.6
 MET-185   ASN-186  9.4 8.9 6.6 -5.6 102.6 103.8 -21.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-209   LYS-210  7.1 7.6 -3.1 -4.8 46.2 50.0 34.4
 LYS-210   VAL-211  8.9 9.3 0.6 -7.9 71.1 73.9 -62.6
 VAL-211   MET-212  12.2 12.8 10.9 -3.4 55.2 43.3 36.0
 MET-212   ASN-213  11.0 11.8 -11.8 10.6 57.1 68.0 -27.1
 ASN-213   ASP-214  10.3 11.0 -12.0 -0.8 57.4 56.7 60.0
 ASP-214   LEU-215  13.3 13.6 -12.7 4.4 124.4 122.4 -24.3
 LEU-215   VAL-216  15.4 16.4 1.1 -18.8 84.3 61.3 11.0
 VAL-216   ASP-217  14.2 15.8 3.4 0.6 142.3 125.3 -10.5
 ASP-217   LYS-218  15.5 15.8 -5.5 6.0 96.5 70.3 17.7
 LYS-218   ILE-219  18.8 18.8 -5.8 2.9 129.2 134.2 -26.9
 ILE-219   ILE-220  19.3 20.6 -0.2 5.8 64.4 94.9 19.7
 ILE-220   ALA-221  19.1 20.1 -2.9 0.2 48.2 42.1 11.6
 ALA-221   ASP-222  21.1 21.4 2.7 1.4 65.8 90.9 4.3
 ASP-222   ARG-223  23.8 24.3 0.9 -8.2 68.3 47.7 -35.6
 ARG-223   LYS-224  24.3 25.3 -0.3 3.7 40.5 74.3 18.1
 LYS-224   ALA-225  24.8 25.5 -20.4 39.9 70.2 53.2 -25.3
 ALA-225   SER-226  27.4 27.5 -54.3 8.6 134.0 120.7 -209.2
 SER-226   GLY-227  29.2 29.4 -162.4 -168.4 81.4 122.2 -36.0
 GLY-227   GLU-228  32.9 30.3 -17.3 172.6 156.7 169.8 1027.5
 GLU-228   GLN-229  33.0 28.9 -83.3 15.7 103.1 128.7 -255.4
 GLN-229   SER-230  32.2 32.1 -79.7 -12.5 141.5 150.4 -522.6
 SER-230   ASP-231  29.0 29.9 37.1 -9.4 70.4 52.9 109.1
 ASP-231   ASP-232  28.0 28.4 -12.9 7.1 129.2 117.7 -17.6
 ASP-232   LEU-233  24.6 24.8 -16.3 2.0 79.1 78.0 51.6
 LEU-233   LEU-234  21.0 21.4 7.9 -4.8 165.0 162.1 -33.6
 LEU-234   THR-235  20.8 21.5 -1.9 2.8 69.0 76.4 4.5
 THR-235   HIS-236  24.1 25.1 -6.1 3.9 99.7 108.4 4.1
 HIS-236   MET-237  23.0 24.2 4.1 -14.8 123.6 122.3 29.1
 MET-237   LEU-238  19.7 21.4 11.2 -0.7 156.2 153.1 -64.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees