Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-71   ILE-72  1.5 1.4 -1.2 -19.8 10.5 16.0 210.7  ILE-72   ARG-73  3.3 3.6 -37.2 47.3 86.3 79.0 -145.4  ARG-73   THR-74  5.9 6.1 -2.5 -0.5 15.5 23.4 136.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-166   LEU-167  9.3 9.5 -6.0 -5.5 84.3 75.8 0.3  LEU-167   PRO-168  6.2 6.6 -18.0 6.7 50.4 41.9 73.5  PRO-168   ALA-169  8.1 8.6 8.0 -27.7 153.9 144.0 129.0  ALA-169   LEU-170  7.9 7.6 -7.7 33.9 79.0 75.8 -76.3  LEU-170   ALA-171  7.5 8.3 -30.5 21.1 147.3 148.9 -140.3  ALA-171   GLU-172  6.8 7.4 -40.9 11.6 92.2 98.0 24.6  GLU-172   LYS-173  10.3 11.0 40.3 -36.0 132.6 106.1 72.4  LYS-173   ASP-174  10.7 11.2 0.1 -2.3 158.6 169.1 90.4  ASP-174   LYS-175  9.0 9.3 -7.5 26.0 67.7 68.2 -91.1  LYS-175   GLN-176  12.1 12.2 -15.2 9.8 77.5 65.5 -14.6  GLN-176   ASP-177  14.1 14.4 5.1 7.4 153.0 150.7 -64.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees