Glutamate Receptor Subunit 2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-105   PHE-106  8.6 8.6 -3.0 6.1 127.6 130.7 -1.9  PHE-106   MET-107  10.0 10.0 0.9 5.9 53.2 56.5 17.6  MET-107   SER-108  6.6 6.6 -9.7 0.2 65.2 57.8 19.3  SER-108   LEU-109  3.1 3.5 -1.7 1.2 132.9 129.4 -4.8  LEU-109   GLY-110  0.7 0.3 11.4 -14.9 121.5 122.6 5.0  GLY-110   ILE-111  4.1 3.8 27.0 -12.1 47.7 49.5 53.0  ILE-111   SER-112  7.5 7.3 -5.4 0.6 113.9 113.9 2.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-216   SER-217  6.8 6.4 6.6 -2.5 100.6 101.4 3.1  SER-217   LYS-218  3.0 2.6 9.7 3.6 70.1 80.9 27.4  LYS-218   GLY-219  2.5 2.6 -25.3 24.3 54.3 61.4 -2.6  GLY-219   TYR-220  4.5 4.5 4.8 -20.9 102.3 115.5 39.5  TYR-220   GLY-221  7.8 7.5 -1.3 -1.8 121.6 116.2 6.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-253   TRP-254  12.0 11.9 -13.3 10.6 115.5 117.2 -17.6  TRP-254   TRP-255  8.7 8.7 -5.0 13.2 91.2 94.3 -14.6  TRP-255   TYR-256  6.7 6.3 -57.3 -43.0 118.4 125.4 -254.7  TYR-256   ASP-257  9.4 9.0 -175.7 43.0 134.2 139.7 1046.3  ASP-257   LYS-258  8.5 11.6 -140.3 -6.6 143.7 141.8 -672.3  LYS-258   GLY-259  9.6 8.9 -40.1 -92.7 121.1 99.6 -201.1  GLY-259   GLU-260  9.8 6.9 109.7 -50.3 39.3 18.9 254.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees