Isocitrate Dehydrogenase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-125   ILE-126  3.6 3.5 -4.5 12.4 34.8 34.9 -12.4  ILE-126   CYS-127  3.1 3.0 -3.9 4.8 32.3 41.2 22.2  CYS-127   LEU-128  3.3 3.3 -4.3 2.9 54.2 49.6 -8.9  LEU-128   ARG-129  2.7 2.4 -2.9 -2.8 17.5 16.6 24.9  ARG-129   PRO-130  3.1 3.0 4.0 2.8 127.9 126.2 -27.5  PRO-130   VAL-131  4.7 4.5 6.2 6.3 156.2 151.9 -67.5  VAL-131   ARG-132  5.3 5.5 -9.5 13.0 50.5 49.3 -42.7  ARG-132   TYR-133  6.7 6.2 -2.5 -16.2 27.6 24.9 122.3  ARG-132   TYR-133  6.7 6.2 -2.5 -16.2 27.6 24.9 122.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-132   TYR-133  6.7 6.2 -2.5 -16.2 27.6 24.9 122.3  ARG-132   TYR-133  6.7 6.2 -2.5 -16.2 27.6 24.9 122.3  TYR-133   TYR-134  6.2 6.0 -18.1 24.3 69.1 64.2 -15.9  TYR-134   GLN-135  5.7 5.3 8.8 -7.3 171.6 164.3 0.0  GLN-135   GLY-136  7.0 6.8 10.9 -16.2 122.4 123.6 31.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-322   ALA-323  4.0 3.9 -21.0 8.8 41.5 40.3 49.7  ALA-323   PRO-324  2.5 2.2 -2.6 -2.3 37.8 35.8 25.0  PRO-324   GLY-325  2.5 2.6 1.7 6.2 145.5 146.6 -34.8  GLY-325   ALA-326  1.9 2.1 -2.4 13.3 35.5 38.7 -40.9  ALA-326   ASN-327  2.5 2.7 0.8 -0.9 141.6 137.5 48.6  ASN-327   ILE-328  2.5 2.7 2.7 -5.8 131.7 135.7 -20.8  ILE-328   GLY-329  1.9 2.0 0.4 15.0 156.2 155.3 17.0  GLY-329   ASP-330  1.7 2.0 -11.7 16.2 62.1 81.5 8.4  ASP-330   GLU-331  4.0 5.0 12.4 -36.2 93.7 87.7 25.8  GLU-331   CYS-332  6.5 7.0 27.5 16.3 32.5 58.3 183.3  CYS-332   ALA-333  7.2 6.8 -25.0 -7.0 142.9 154.2 -152.4  ALA-333   LEU-334  6.1 6.5 -17.6 1.2 125.5 124.2 -45.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees