Protein (Arrestin)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-175   LYS-176  5.0 4.3 -6.7 15.8 54.6 54.1 -16.5  LYS-176   VAL-177  3.7 3.6 12.4 -24.1 148.4 145.9 19.3  VAL-177   GLN-178  3.8 3.4 0.0 -25.7 40.0 37.5 147.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-294   LEU-295  11.1 11.5 8.6 -12.0 82.9 74.9 1.4  LEU-295   ASP-296  9.6 9.9 -15.4 35.7 71.7 74.8 -46.6  ASP-296   GLY-297  6.5 6.9 129.3 94.8 145.6 156.0 559.2  GLY-297   LYS-298  8.5 6.3 -45.6 -10.4 63.0 79.6 -12.8  LYS-298   ILE-299  11.1 9.0 6.5 -20.3 59.2 127.9 -81.2  ILE-299   LYS-300  14.6 9.4 -24.2 -78.7 79.8 32.4 -790.6  LYS-300   HIS-301  16.4 11.9 76.8 172.5 75.3 71.8 -186.9  HIS-301   GLU-302  15.0 14.2 -130.6 -17.5 160.1 144.4 842.3  GLU-302   ASP-303  16.7 15.2 -127.6 -12.3 131.8 133.7 -620.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-317   LYS-318  16.7 20.4 -168.6 -47.1 89.4 57.5 -84.2  LYS-318   THR-319  14.3 18.1 166.1 -8.2 149.1 65.3 322.2  THR-319   VAL-320  14.8 16.4 162.4 -12.6 123.6 114.9 -349.5  VAL-320   MET-321  12.6 12.7 133.6 -14.9 74.0 112.7 159.0  MET-321   GLY-322  9.8 12.1 179.8 30.0 119.9 86.5 317.0  GLY-322   ILE-323  10.2 9.8 -76.6 21.1 118.0 135.6 -175.1  ILE-323   LEU-324  10.3 9.4 -11.7 -15.0 163.4 148.4 -94.7  LEU-324   VAL-325  10.8 9.2 3.3 2.1 60.8 45.7 41.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees