Saccharopine Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LEU-181   ALA-182  23.6 24.7 -9.4 -19.5 50.7 44.7 287.2
 ALA-182   LEU-183  20.2 21.4 18.6 2.0 107.9 103.6 -134.8
 LEU-183   ARG-184  21.4 21.5 -11.2 31.9 54.5 44.5 -278.3
 ARG-184   ASN-185  24.3 24.3 -26.2 24.9 27.9 26.6 80.6
 ASN-185   ALA-186  22.3 21.8 -20.9 9.2 52.0 62.5 68.0
 ALA-186   ALA-187  21.3 20.8 -17.7 22.7 87.5 86.2 -43.2
 ALA-187   SER-188  17.8 17.3 -10.9 11.1 27.7 30.1 -18.9
 SER-188   PHE-189  16.1 15.9 0.7 1.6 86.7 91.7 -5.1
 PHE-189   TYR-190  13.3 13.3 -3.5 4.7 47.1 47.0 -3.1
 TYR-190   LYS-191  12.6 12.8 3.7 -0.3 61.2 62.8 37.4
 LYS-191   ASP-192  15.2 15.5 9.8 -10.6 140.2 142.1 12.7
 ASP-192   GLY-193  16.3 16.7 3.7 -13.1 155.1 150.1 125.5
 GLY-193   LYS-194  14.0 14.2 9.6 3.4 107.6 107.1 -59.3
 LYS-194   VAL-195  15.3 15.9 -7.1 1.4 131.6 133.4 -103.5
 VAL-195   THR-196  15.4 15.9 8.1 -11.3 62.3 58.2 -43.5
 LEU-181   ALA-182  13.8 14.9 -9.4 -19.5 129.0 135.1 -291.8
 LEU-181   ALA-182  8.7 10.1 -9.4 -19.5 88.4 84.5 58.7
 ALA-182   LEU-183  5.4 7.4 18.6 2.0 45.2 60.6 167.2
 LEU-183   ARG-184  4.0 5.0 -11.2 31.9 161.7 161.2 258.1
 ARG-184   ASN-185  5.1 5.9 -26.2 24.9 94.8 91.4 -116.2
 ASN-185   ALA-186  5.2 6.0 -20.9 9.2 110.6 105.1 -96.4
 ALA-186   ALA-187  5.2 5.4 -17.7 22.7 37.8 38.5 -42.2
 ALA-187   SER-188  6.3 6.0 -10.9 11.1 112.6 117.5 -21.9
 SER-188   PHE-189  9.8 9.5 0.7 1.6 138.3 132.8 -15.6
 PHE-189   TYR-190  11.7 11.6 -3.5 4.7 114.8 118.1 -3.4
 TYR-190   LYS-191  15.0 14.9 3.7 -0.3 89.7 87.9 -15.8
 LYS-191   ASP-192  15.5 15.4 9.8 -10.6 56.0 55.4 -21.6
 ASP-192   GLY-193  19.1 19.0 3.7 -13.1 74.0 76.7 -60.9
 GLY-193   LYS-194  20.3 20.0 9.6 3.4 56.7 51.6 71.9
 LYS-194   VAL-195  18.0 17.6 -7.1 1.4 77.0 75.2 37.5
 LEU-181   ALA-182  23.6 24.7 -9.4 -19.5 50.7 44.7 287.2
 ALA-182   LEU-183  20.2 21.4 18.6 2.0 107.9 103.6 -134.8
 LYS-171   PHE-172  2.3 2.2 -2.1 12.3 116.2 122.8 25.5
 PHE-172   SER-173  5.1 4.8 -17.7 27.1 119.9 130.2 49.1
 SER-173   TRP-174  7.8 6.7 -37.9 39.2 94.2 91.4 -56.0
 TRP-174   SER-175  10.2 9.1 -33.3 16.1 33.7 22.9 252.1
 SER-175   SER-176  11.5 11.1 -10.2 12.3 75.6 64.2 2.9
 SER-176   ARG-177  9.0 9.1 -7.2 7.9 84.3 74.5 7.0
 ARG-177   GLY-178  12.1 12.6 -167.7 171.6 148.6 134.0 66.3
 GLY-178   VAL-179  12.4 12.9 -1.6 16.2 89.8 87.9 -11.8
 VAL-179   LEU-180  9.1 9.9 -18.4 18.5 30.9 43.3 -40.2
 LEU-180   LEU-181  10.1 11.2 -15.2 20.9 88.5 97.1 -51.4
 LEU-181   ALA-182  13.8 14.9 -9.4 -19.5 129.0 135.1 -291.8
 LYS-171   PHE-172  12.2 12.4 -2.1 12.3 96.2 95.1 48.7
 PHE-172   SER-173  12.9 12.3 -17.7 27.1 35.1 35.2 -75.1
 SER-173   TRP-174  13.7 12.2 -37.9 39.2 110.0 115.0 76.9
 TRP-174   SER-175  15.1 13.1 -33.3 16.1 161.2 149.9 -186.7
 SER-175   SER-176  15.8 14.9 -10.2 12.3 111.4 124.9 30.4
 SER-176   ARG-177  14.0 13.7 -7.2 7.9 147.9 156.3 6.0
 ARG-177   GLY-178  13.7 14.5 -167.7 171.6 78.8 94.4 -102.3
 GLY-178   VAL-179  10.5 12.2 -1.6 16.2 80.9 96.1 -76.5
 VAL-179   LEU-180  8.5 9.2 -18.4 18.5 143.6 158.3 -16.4
 LEU-180   LEU-181  10.0 10.0 -15.2 20.9 143.8 134.2 98.4
 LEU-181   ALA-182  8.7 10.1 -9.4 -19.5 88.4 84.5 58.7
 ALA-182   LEU-183  5.4 7.4 18.6 2.0 45.2 60.6 167.2
 LEU-181   ALA-182  23.6 24.7 -9.4 -19.5 50.7 44.7 287.2
 ALA-182   LEU-183  20.2 21.4 18.6 2.0 107.9 103.6 -134.8
 LEU-183   ARG-184  21.4 21.5 -11.2 31.9 54.5 44.5 -278.3
 ARG-184   ASN-185  24.3 24.3 -26.2 24.9 27.9 26.6 80.6
 ASN-185   ALA-186  22.3 21.8 -20.9 9.2 52.0 62.5 68.0
 ALA-186   ALA-187  21.3 20.8 -17.7 22.7 87.5 86.2 -43.2
 ALA-187   SER-188  17.8 17.3 -10.9 11.1 27.7 30.1 -18.9
 SER-188   PHE-189  16.1 15.9 0.7 1.6 86.7 91.7 -5.1
 PHE-189   TYR-190  13.3 13.3 -3.5 4.7 47.1 47.0 -3.1
 TYR-190   LYS-191  12.6 12.8 3.7 -0.3 61.2 62.8 37.4
 LYS-191   ASP-192  15.2 15.5 9.8 -10.6 140.2 142.1 12.7
 ASP-192   GLY-193  16.3 16.7 3.7 -13.1 155.1 150.1 125.5
 GLY-193   LYS-194  14.0 14.2 9.6 3.4 107.6 107.1 -59.3
 LYS-194   VAL-195  15.3 15.9 -7.1 1.4 131.6 133.4 -103.5
 VAL-195   THR-196  15.4 15.9 8.1 -11.3 62.3 58.2 -43.5
 LYS-171   PHE-172  2.3 2.2 -2.1 12.3 116.2 122.8 25.5
 PHE-172   SER-173  5.1 4.8 -17.7 27.1 119.9 130.2 49.1
 SER-173   TRP-174  7.8 6.7 -37.9 39.2 94.2 91.4 -56.0
 TRP-174   SER-175  10.2 9.1 -33.3 16.1 33.7 22.9 252.1
 SER-175   SER-176  11.5 11.1 -10.2 12.3 75.6 64.2 2.9
 SER-176   ARG-177  9.0 9.1 -7.2 7.9 84.3 74.5 7.0
 ARG-177   GLY-178  12.1 12.6 -167.7 171.6 148.6 134.0 66.3
 GLY-178   VAL-179  12.4 12.9 -1.6 16.2 89.8 87.9 -11.8
 VAL-179   LEU-180  9.1 9.9 -18.4 18.5 30.9 43.3 -40.2
 LEU-180   LEU-181  10.1 11.2 -15.2 20.9 88.5 97.1 -51.4
 LEU-181   ALA-182  13.8 14.9 -9.4 -19.5 129.0 135.1 -291.8
 LYS-171   PHE-172  12.2 12.4 -2.1 12.3 96.2 95.1 48.7
 PHE-172   SER-173  12.9 12.3 -17.7 27.1 35.1 35.2 -75.1
 SER-173   TRP-174  13.7 12.2 -37.9 39.2 110.0 115.0 76.9
 TRP-174   SER-175  15.1 13.1 -33.3 16.1 161.2 149.9 -186.7
 SER-175   SER-176  15.8 14.9 -10.2 12.3 111.4 124.9 30.4
 SER-176   ARG-177  14.0 13.7 -7.2 7.9 147.9 156.3 6.0
 ARG-177   GLY-178  13.7 14.5 -167.7 171.6 78.8 94.4 -102.3
 GLY-178   VAL-179  10.5 12.2 -1.6 16.2 80.9 96.1 -76.5
 VAL-179   LEU-180  8.5 9.2 -18.4 18.5 143.6 158.3 -16.4
 LEU-180   LEU-181  10.0 10.0 -15.2 20.9 143.8 134.2 98.4
 LEU-181   ALA-182  8.7 10.1 -9.4 -19.5 88.4 84.5 58.7
 ALA-182   LEU-183  5.4 7.4 18.6 2.0 45.2 60.6 167.2
 LEU-183   ARG-184  4.0 5.0 -11.2 31.9 161.7 161.2 258.1
 ARG-184   ASN-185  5.1 5.9 -26.2 24.9 94.8 91.4 -116.2
 ASN-185   ALA-186  5.2 6.0 -20.9 9.2 110.6 105.1 -96.4
 ALA-186   ALA-187  5.2 5.4 -17.7 22.7 37.8 38.5 -42.2
 ALA-187   SER-188  6.3 6.0 -10.9 11.1 112.6 117.5 -21.9
 SER-188   PHE-189  9.8 9.5 0.7 1.6 138.3 132.8 -15.6
 PHE-189   TYR-190  11.7 11.6 -3.5 4.7 114.8 118.1 -3.4
 TYR-190   LYS-191  15.0 14.9 3.7 -0.3 89.7 87.9 -15.8
 LYS-191   ASP-192  15.5 15.4 9.8 -10.6 56.0 55.4 -21.6
 ASP-192   GLY-193  19.1 19.0 3.7 -13.1 74.0 76.7 -60.9
 GLY-193   LYS-194  20.3 20.0 9.6 3.4 56.7 51.6 71.9
 LYS-194   VAL-195  18.0 17.6 -7.1 1.4 77.0 75.2 37.5

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees