Preprotein Translocase Seca Subunit

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-223   SER-224  3.4 1.2 -6.9 53.6 162.5 153.4 55.2  SER-224   GLY-225  2.5 1.3 3.0 117.5 64.2 82.3 23.2  GLY-225   GLN-226  2.0 4.0 43.9 20.7 72.2 47.1 95.9  GLN-226   ALA-227  2.1 6.4 -141.3 52.4 110.4 157.9 -63.7  ALA-227   ALA-228  5.8 5.7 100.3 0.2 62.9 65.4 55.1  ALA-228   LYS-229  7.3 3.1 119.3 -103.9 41.3 83.9 24.4  LYS-229   SER-230  8.6 2.8 87.5 -1.5 38.9 12.5 -332.1  SER-230   THR-231  6.0 4.8 -121.4 -67.0 167.1 116.8 181.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-243   LEU-244  21.6 18.1 -61.6 15.7 155.4 102.0 -27.2  LEU-244   LYS-245  22.4 21.0 94.6 -14.1 98.7 128.5 -62.8  LYS-245   ALA-246  26.1 23.8 -150.0 7.4 142.4 123.9 297.1  ALA-246   GLU-247  27.4 25.5 -11.5 -1.4 159.1 115.5 -5.7  GLU-247   LYS-248  25.2 29.0 -14.5 -2.6 96.9 101.2 -3.0  LYS-248   ASP-249  25.0 29.0 -129.0 -42.7 128.5 150.5 -198.9  ASP-249   TYR-250  26.7 26.5 173.0 -90.6 89.4 36.3 90.1  TYR-250   THR-251  29.5 26.6 0.7 -67.0 75.1 88.4 -8.4  THR-251   TYR-252  29.4 28.9 -23.5 49.2 163.6 135.3 27.9  TYR-252   ASP-253  29.8 29.0 -84.2 49.8 76.5 54.4 15.4  TYR-252   ASP-253  29.8 29.0 -84.2 49.8 76.5 54.4 15.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-252   ASP-253  29.8 29.0 -84.2 49.8 76.5 54.4 15.4  TYR-252   ASP-253  29.8 29.0 -84.2 49.8 76.5 54.4 15.4  ASP-253   ILE-254  31.0 31.0 -46.9 -115.8 52.6 60.2 118.4  ILE-254   LYS-255  30.1 33.5 98.2 5.4 70.3 175.3 -97.6  LYS-255   THR-256  26.6 33.8 -6.3 85.5 119.8 60.7 -6.0  THR-256   LYS-257  24.8 30.0 -152.1 -119.0 75.0 105.4 -89.0  LYS-257   ALA-258  25.1 29.1 -53.6 -43.1 148.6 108.0 216.4  ALA-258   VAL-259  24.3 26.2 177.6 -83.8 104.2 33.8 -141.4  VAL-259   GLN-260  23.9 25.1 173.3 -65.2 57.7 103.0 56.0  GLN-260   LEU-261  26.7 25.6 -2.3 92.1 150.3 141.0 103.5  LEU-261   THR-262  26.3 25.5 15.9 -11.9 74.4 90.3 -12.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-346   ASN-347  4.9 5.6 26.3 93.1 40.2 35.9 128.0  ASN-347   GLU-348  2.2 3.0 53.0 -0.5 71.8 23.8 33.6  GLU-348   SER-349  2.7 3.3 -152.0 38.7 169.4 119.4 -118.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees