Dehydroascorbate Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-23   GLN-24  2.2 2.5 7.2 -2.2 77.6 75.7 25.6  GLN-24   ARG-25  5.8 6.1 5.1 -1.2 47.7 49.3 12.5  ARG-25   VAL-26  7.4 7.5 -1.6 0.9 156.0 156.9 -5.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-27   LEU-28  6.5 6.6 -2.8 7.4 77.4 76.9 -18.6  LEU-28   THR-29  9.7 9.9 -3.3 -1.2 22.9 18.9 17.5  THR-29   LEU-30  9.2 9.2 6.1 -3.7 135.7 132.9 -12.0  LEU-30   GLU-31  8.1 8.1 -3.0 6.6 91.1 89.3 -2.4  GLU-31   GLU-32  11.5 11.5 -1.9 -3.4 65.8 59.1 1.6  GLU-32   LYS-33  13.5 13.4 3.7 -6.0 173.7 172.6 22.8  LYS-33   LYS-34  12.0 11.8 7.5 -13.6 119.1 110.0 13.1  LYS-34   VAL-35  12.8 12.5 1.1 6.2 117.2 116.2 -22.5  VAL-35   PRO-36  9.3 9.1 -2.4 2.4 115.1 121.0 2.0  PRO-36   TYR-37  10.1 9.9 3.0 -0.4 67.1 63.7 11.3  TYR-37   GLU-38  8.2 8.1 -7.4 -17.7 123.7 125.6 -61.4  GLU-38   MET-39  9.4 9.5 -2.3 10.4 142.0 134.7 41.4  MET-39   LYS-40  8.4 8.2 -3.0 1.4 116.9 107.3 8.8  LYS-40   LEU-41  9.2 9.3 -3.2 9.3 160.1 151.7 14.4  LEU-41   ILE-42  9.1 9.1 11.0 9.6 70.8 81.8 26.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-62   PHE-63  4.6 4.4 -5.9 1.2 134.3 133.0 -82.2  PHE-63   ASN-64  2.8 2.9 4.7 19.9 31.1 37.9 115.0  ASN-64   GLY-65  4.2 4.8 -1.8 -39.7 131.3 140.5 -164.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees