Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  15.7 15.2 -2.4 5.3 102.7 105.2 22.1
 TYR-166   ARG-167  16.0 15.5 4.5 -18.9 35.3 35.9 -7.6
 ARG-167   ASP-168  13.6 13.1 13.0 -10.5 39.2 35.5 -76.1
 ASP-168   GLN-169  14.1 11.9 -35.5 26.4 112.0 89.6 -12.6
 GLN-169   PRO-170  10.3 8.7 -39.6 -1.0 35.6 13.9 160.7
 PRO-170   HIS-171  7.7 7.0 2.6 17.4 126.4 121.5 -26.8
 HIS-171   PRO-172  6.5 6.3 -3.5 6.0 112.7 109.1 5.7
 PRO-172   PHE-173  4.4 4.8 -1.7 -10.2 66.5 69.8 8.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.3 4.8 10.5 -3.2 68.1 62.1 -6.1
 ALA-225   SER-226  8.5 8.1 -40.7 20.2 112.6 118.3 -52.3
 GLN-229   SER-230  7.1 5.6 -9.3 9.2 45.2 54.5 -16.5
 SER-230   ASP-231  7.0 7.8 15.1 53.2 75.0 95.0 -50.6
 ASP-231   ASP-232  8.1 10.1 -79.7 56.0 31.7 38.1 113.5
 ASP-232   LEU-233  8.5 8.1 -18.3 -15.8 74.9 103.4 3.5
 LEU-233   LEU-234  8.2 7.9 19.9 1.6 78.9 73.6 28.2
 LEU-234   THR-235  4.5 4.2 0.2 -11.1 17.1 4.3 -35.6
 THR-235   HIS-236  4.4 4.5 -2.5 12.2 105.4 115.8 15.2
 HIS-236   MET-237  7.7 7.6 -6.1 -10.2 63.5 82.1 6.2
 MET-237   LEU-238  7.4 7.3 3.0 19.5 57.0 56.8 -5.8
 LEU-238   ASN-239  5.5 5.0 -4.7 25.6 164.8 160.5 93.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees