Vitamin B12 Transport Protein Btuf

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-149   TYR-150  13.5 14.0 11.8 -19.2 88.9 94.0 -34.1  TYR-150   ALA-151  11.8 12.3 16.2 -41.3 146.9 157.7 258.0  ALA-151   ASP-152  13.6 13.7 37.4 -2.3 88.7 91.0 -50.2  ASP-152   LYS-153  16.7 17.0 -29.5 28.3 126.3 115.0 -14.6  LYS-153   PRO-154  15.2 16.0 22.6 -21.9 122.1 131.7 -15.7  PRO-154   LYS-155  16.7 17.8 -115.9 136.4 87.2 72.5 -94.7  LYS-155   LYS-156  14.4 14.6 -57.5 48.3 117.6 116.6 -6.6  LYS-156   ARG-157  14.2 14.3 -5.4 -0.6 60.1 60.7 29.5  ARG-157   VAL-158  14.6 14.5 7.5 -3.4 55.6 52.8 13.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  THR-240   SER-241  7.5 6.8 5.9 -14.3 27.3 27.1 -48.2  SER-241   ASP-242  10.2 9.5 11.4 -5.0 53.7 63.2 39.8  ASP-242   TRP-243  11.2 10.6 11.0 -14.6 65.7 65.1 13.7  TRP-243   PHE-244  7.9 7.4 0.9 -3.4 40.7 37.0 -2.9  PHE-244   GLU-245  6.2 5.7 9.1 -13.5 39.2 40.9 -6.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-251   ILE-252  6.4 6.1 -0.6 -2.3 112.5 110.0 -11.7  ILE-252   LEU-253  6.6 6.4 3.5 6.5 19.2 13.5 69.7  LEU-253   ALA-254  5.9 5.9 -8.4 3.1 91.4 94.0 10.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees