Phosphoenolpyruvate-Protein Phosphotransferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-21   ASP-22  6.0 3.8 124.2 -11.8 58.4 22.4 151.0  ASP-22   GLU-23  3.9 2.4 7.9 40.4 75.9 51.1 30.4  GLU-23   ILE-24  1.3 2.3 33.9 -65.3 53.7 73.5 -32.2  ILE-24   VAL-25  2.3 2.4 -10.0 21.2 172.2 167.9 28.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-144   LYS-145  6.8 6.0 21.6 -17.2 3.2 9.3 -6.2  LYS-145   ILE-146  6.9 5.6 -8.5 35.9 115.9 123.6 29.5  ILE-146   ILE-147  4.6 3.9 36.2 41.0 26.0 45.3 108.8  ILE-147   ASP-148  4.0 1.5 159.4 152.9 55.3 19.4 -70.9  ASP-148   LEU-149  2.1 1.8 4.5 -36.6 47.3 61.5 -20.8  LEU-149   SER-150  2.7 1.9 29.4 5.3 11.7 21.9 56.0  SER-150   ALA-151  0.9 2.2 16.6 53.2 69.1 93.0 -13.7  ALA-151   ILE-152  4.0 5.6 -66.6 32.4 63.4 109.6 28.8  ILE-152   GLN-153  6.5 7.1 -3.4 -50.6 135.2 130.0 -29.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees