Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-17   PRO-18  7.3 7.1 -4.5 -6.2 87.2 90.1 0.9
 PRO-18   LEU-19  10.2 10.1 3.1 23.3 27.4 25.7 120.2
 LEU-19   LEU-20  10.6 10.4 -19.5 31.9 113.8 109.0 19.5
 LEU-20   ASN-21  11.3 11.6 -43.2 5.6 50.2 55.2 170.1
 ASN-21   THR-22  14.3 14.1 -1.5 -3.0 123.1 115.6 -67.1
 THR-22   ASP-23  16.8 16.8 0.8 12.4 79.2 64.4 34.9
 ASP-23   LYS-24  18.9 19.6 -22.8 -0.6 174.4 162.0 -102.9
 LYS-24   PRO-25  17.7 18.0 -3.2 8.3 96.3 96.5 8.9
 PRO-25   VAL-26  14.0 14.5 -5.9 -7.0 155.9 154.0 -56.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLU-38   ILE-39  1.9 2.1 2.4 4.3 42.7 42.8 7.1
 ILE-39   PHE-40  0.7 0.7 4.2 4.6 32.7 30.9 29.2
 PHE-40   LYS-41  1.3 1.3 3.9 -1.3 45.1 44.2 32.8

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-47   VAL-48  6.9 7.2 12.4 -15.4 46.7 47.9 -48.6
 VAL-48   THR-49  5.5 5.7 2.3 17.3 30.6 29.3 101.0
 THR-49   ARG-50  5.9 6.0 10.9 -8.9 49.5 53.9 -57.0
 ARG-50   TYR-51  4.1 4.0 13.6 -7.5 41.4 44.7 20.9

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees