Fab Fragment (Light Chain)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-103   GLU-104  3.6 3.7 -19.5 9.7 87.5 91.2 5.5
 GLU-104   ILE-105  1.3 1.0 -0.8 24.0 160.2 157.0 90.7
 ILE-105   LEU-106  2.2 2.3 -47.6 42.1 105.5 105.4 -10.0
 LEU-106   ARG-107  4.8 4.7 41.7 -36.7 54.8 58.2 21.2
 ARG-107   ALA-108  7.3 7.5 7.6 1.7 76.0 76.9 2.0

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees