Dna-Directed RNA Polymerase II Largest Subunit

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ARG-344   VAL-345  20.0 19.9 45.2 -25.0 46.5 38.0 42.3
 VAL-345   ASP-346  19.4 18.0 78.7 0.3 18.4 32.7 229.8
 ASP-346   PHE-347  19.0 19.0 22.9 -11.1 48.3 51.4 28.7
 PHE-347   SER-348  18.5 18.8 5.1 23.5 100.6 111.0 -17.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LEU-1381   THR-1382  20.9 20.6 0.4 -2.1 94.5 96.8 -10.1
 THR-1382   SER-1383  17.8 17.6 2.7 -1.2 124.4 122.5 -14.6
 SER-1383   VAL-1384  19.0 18.6 -6.1 23.7 38.6 32.9 -35.8
 VAL-1384   THR-1385  18.1 18.2 36.6 -18.8 89.2 73.5 1.0
 THR-1385   ARG-1386  19.4 17.5 116.8 -121.7 61.2 87.1 8.2
 ARG-1386   HIS-1387  16.1 14.2 -130.4 33.5 165.7 137.3 -293.8
 HIS-1387   GLY-1388  14.3 13.8 52.5 -37.0 79.4 104.8 -3.0
 GLY-1388   PHE-1389  10.9 13.9 140.9 3.6 108.2 133.3 591.2
 PHE-1389   ASN-1390  11.2 12.3 6.8 55.9 131.4 99.3 -28.4
 ASN-1390   ARG-1391  8.0 9.6 -122.9 174.7 70.9 128.0 -42.3
 ARG-1391   SER-1392  7.2 8.0 128.9 -153.5 99.0 116.0 -79.9
 SER-1392   ASN-1393  9.5 8.9 5.8 -47.8 124.0 94.9 -17.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLN-1432   MET-1433  3.9 3.8 -7.6 33.7 94.5 100.8 6.1
 MET-1433   ALA-1434  0.5 1.2 22.7 -69.0 134.8 148.9 116.4
 ALA-1434   PRO-1435  2.3 2.9 31.8 -5.2 73.6 76.4 29.7
 PRO-1435   ILE-1436  5.4 6.2 15.8 14.7 88.1 76.7 26.3
 ILE-1436   GLY-1437  7.6 8.0 30.2 -82.1 20.9 36.6 -170.6
 GLY-1437   THR-1438  10.0 10.1 49.7 -3.5 41.6 56.6 96.0
 THR-1438   GLY-1439  7.9 7.0 -8.2 -5.4 75.2 62.0 7.1
 GLY-1439   ALA-1440  5.8 5.6 33.6 -29.5 132.2 131.6 -22.5
 ALA-1440   PHE-1441  8.8 8.9 -73.8 73.9 110.6 113.5 15.0
 PHE-1441   ASP-1442  10.1 10.3 -16.4 9.6 123.6 112.4 -6.9
 ASP-1442   VAL-1443  9.6 9.2 -10.0 7.6 157.4 162.8 -1.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees