Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-18   LEU-19  12.4 12.0 6.4 26.8 15.0 16.7 176.1  LEU-19   LEU-20  12.0 11.7 -21.3 35.2 114.1 111.5 35.5  LEU-20   ASN-21  12.1 12.5 -42.8 8.3 58.2 65.6 104.6  ASN-21   THR-22  15.4 15.6 0.1 -0.5 47.0 49.6 -47.8  THR-22   ASP-23  17.2 17.7 -7.4 20.8 96.5 114.6 -11.5  ASP-23   LYS-24  18.8 20.1 -18.6 -2.0 162.1 150.2 -51.1  LYS-24   PRO-25  17.1 17.7 -1.7 3.0 87.3 84.5 -7.9  PRO-25   VAL-26  13.4 14.2 -2.9 -10.3 144.2 143.1 -33.9  VAL-26   GLN-27  12.3 12.8 8.8 -1.7 56.5 57.8 38.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-35   LEU-36  10.4 10.6 1.3 -3.2 79.6 82.1 -27.5  LEU-36   GLY-37  7.7 7.9 -2.6 5.3 50.1 51.2 -23.3  GLY-37   GLU-38  5.5 5.7 -4.1 -4.8 99.6 97.0 2.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-50   TYR-51  3.0 2.9 3.4 -2.7 32.9 35.2 -8.2  TYR-51   LEU-52  2.8 2.7 -7.4 8.0 148.3 149.0 28.3  LEU-52   SER-53  2.9 2.7 -4.2 5.0 136.9 138.2 -25.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees