Aspartate Aminotransferase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-34   LEU-35  12.6 12.8 9.2 -20.7 105.3 103.7 49.6  LEU-35   GLY-36  9.6 9.8 23.8 6.8 127.8 138.9 -169.2  GLY-36   VAL-37  10.5 10.5 -103.6 12.2 118.8 96.6 -141.4  VAL-37   GLY-38  9.9 10.3 -3.5 31.7 66.2 120.0 52.0  GLY-38   ALA-39  9.1 7.5 70.3 8.8 48.7 33.4 490.0  ALA-39   TYR-40  8.4 8.5 -33.7 -10.3 129.1 129.7 -203.3  TYR-40   ARG-41  5.9 6.1 3.0 1.9 49.1 46.4 31.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-328   ARG-329  6.7 6.4 -1.6 -0.2 92.5 90.7 20.1  ARG-329   ILE-330  3.6 3.4 3.2 2.0 19.0 24.8 54.0  ILE-330   LEU-331  3.5 3.1 1.4 -0.5 73.8 74.3 -31.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-356   ILE-357  7.1 6.6 -14.0 7.4 150.7 148.0 -93.7  ILE-357   GLY-358  6.6 6.0 26.3 -2.0 81.7 90.2 87.7  GLY-358   MET-359  4.4 4.4 12.7 -16.6 78.5 62.5 73.6  MET-359   PHE-360  1.4 1.9 17.3 -14.2 135.3 121.9 -38.3  PHE-360   SER-361  1.0 1.1 18.7 11.5 117.6 110.4 -141.1  SER-361   PHE-362  2.8 3.7 -7.7 -5.3 38.9 34.6 19.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees