Bifunctional P-450/nadph-P450 Reductase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-165   TYR-166  16.8 16.5 0.5 -0.7 79.4 79.6 -11.5
 TYR-166   ARG-167  17.2 16.9 4.4 -22.1 30.7 32.2 -71.9
 ARG-167   ASP-168  15.1 15.0 19.5 8.5 40.9 36.0 59.7
 ASP-168   GLN-169  15.9 14.1 -47.1 31.3 117.3 90.0 -56.8
 GLN-169   PRO-170  12.1 10.7 -35.8 -4.8 40.4 19.3 157.2
 PRO-170   HIS-171  9.4 8.7 7.5 12.6 124.0 118.7 -24.7
 HIS-171   PRO-172  7.8 7.9 -2.7 5.5 118.1 113.2 -11.6
 PRO-172   PHE-173  5.5 6.0 -11.3 3.3 71.0 71.3 37.4
 PHE-173   ILE-174  2.7 2.9 -6.9 5.1 124.5 120.6 -18.7

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 LYS-224   ALA-225  5.3 4.9 -5.9 13.5 115.0 126.5 8.0
 ALA-225   SER-226  8.2 7.8 -41.2 24.2 117.8 124.5 -65.3
 GLN-229   SER-230  5.8 4.7 -5.5 6.2 47.0 59.2 -29.7
 SER-230   ASP-231  6.1 7.1 14.9 45.2 77.8 97.4 -53.6
 ASP-231   ASP-232  7.6 9.8 -74.1 43.8 32.2 31.5 136.1
 ASP-232   LEU-233  8.3 8.2 -18.2 -10.9 69.4 98.0 7.9
 LEU-233   LEU-234  8.2 8.0 21.6 -8.5 82.3 75.8 10.3
 LEU-234   THR-235  4.6 4.4 2.9 -7.7 22.0 9.0 -27.3
 THR-235   HIS-236  4.5 4.4 -4.0 17.3 100.9 109.7 14.5
 HIS-236   MET-237  8.0 7.9 -11.2 -13.4 58.2 76.8 14.4
 MET-237   LEU-238  7.8 7.8 13.1 18.4 60.0 58.7 12.1
 LEU-238   ASN-239  6.2 5.5 -8.3 24.0 159.6 154.0 74.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees