Pyruvate Kinase Isozymes M1/m2

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-117   GLU-118  7.7 7.0 -6.1 16.8 137.7 140.1 72.8  GLU-118   ILE-119  5.8 5.7 -3.5 0.6 69.5 71.6 44.3  ILE-119   ARG-120  3.9 3.8 4.9 -4.6 53.3 55.5 13.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-209   ASN-210  3.2 3.1 14.6 -3.6 87.6 87.8 30.3  ASN-210   LEU-211  0.7 0.6 8.1 0.4 88.6 83.5 8.3  LEU-211   PRO-212  3.9 4.1 -12.6 8.0 79.7 74.8 20.2  PRO-212   GLY-213  6.5 6.5 -2.0 5.6 109.8 109.3 30.8  GLY-213   ALA-214  9.9 10.0 -10.0 4.1 161.8 169.8 -31.3  ALA-214   ALA-215  8.5 8.8 12.5 -8.2 49.6 58.4 -15.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-216   ASP-217  6.0 5.9 -16.0 12.1 139.6 139.2 3.3  ASP-217   LEU-218  6.5 6.5 -25.9 26.4 106.8 113.2 25.7  LEU-218   PRO-219  5.9 5.0 14.9 2.8 77.3 78.8 23.2  PRO-219   ALA-220  9.0 8.2 -12.8 1.5 45.8 41.6 27.9  ALA-220   VAL-221  12.2 11.3 -1.3 0.2 95.5 101.5 14.7  VAL-221   SER-222  13.0 12.4 -7.4 2.0 128.5 134.9 -6.6  SER-222   GLU-223  10.4 10.0 2.0 -5.8 52.9 53.9 0.8  GLU-223   LYS-224  11.4 11.4 2.5 0.3 121.9 121.4 7.8  LYS-224   ASP-225  12.9 13.0 0.3 -2.2 116.2 121.1 -24.5  ASP-225   ILE-226  14.4 14.2 3.6 -0.3 52.5 53.0 22.8  ILE-226   GLN-227  16.4 16.1 -2.0 1.7 92.5 94.5 -9.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees