Rap Guanine Nucleotide Exchange Factor 4

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASN-436   ARG-437  8.2 9.2 2.1 -13.1 35.3 59.9 -0.4  ARG-437   ILE-438  6.9 6.5 8.3 -33.4 98.7 114.1 10.7  ILE-438   LEU-439  5.3 5.9 -3.5 -45.8 55.3 9.2 24.9  LEU-439   ARG-440  3.3 6.6 39.6 16.0 62.3 126.6 14.2  ARG-440   ASP-441  3.5 6.5 -17.6 -38.7 136.7 48.6 16.2  ASP-441   VAL-442  0.9 3.4 59.5 -53.0 113.4 109.8 -10.2  VAL-442   GLU-443  1.9 3.2 -14.4 -7.5 69.7 52.1 5.4  GLU-443   ALA-444  3.5 6.6 112.0 -34.7 38.1 137.4 16.6  ALA-444   ASN-445  4.7 4.9 23.8 53.1 73.0 158.7 87.1  ASN-445   THR-446  5.4 7.1 127.7 64.4 135.9 112.9 -75.8  THR-446   VAL-447  7.4 8.1 6.2 20.6 83.2 79.2 3.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees