2E8 (Igg1=kappa=) Antibody

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-111   SER-112  8.7 8.6 -1.7 3.1 141.3 140.5 13.8  SER-112   ALA-113  7.9 7.8 -18.6 2.6 94.2 91.0 -82.8  ALA-113   ALA-114  9.1 9.2 10.4 5.8 32.3 26.5 206.4  ALA-114   LYS-115  10.1 10.0 2.0 7.7 93.5 88.2 -10.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees