Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-89   ARG-90  7.7 7.1 -1.0 11.4 60.7 70.5 -65.4  ARG-90   THR-91  9.1 8.7 9.7 -16.6 176.4 160.4 146.3  THR-91   GLY-92  8.7 8.4 5.5 -3.2 124.9 117.6 46.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-183   VAL-184  6.6 7.0 16.2 5.9 52.2 56.4 61.9  VAL-184   ASP-185  5.6 5.9 31.4 -29.1 124.6 103.9 -11.1  ASP-185   LEU-186  9.0 9.6 -34.9 24.2 106.6 106.2 -16.0  LEU-186   PRO-187  9.8 10.3 -12.2 5.0 44.0 44.4 10.5  PRO-187   ALA-188  13.1 13.6 -4.1 -2.9 34.4 36.7 21.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-264   LEU-265  6.3 6.4 0.7 1.9 87.4 78.0 62.6  LEU-265   GLY-266  4.1 4.1 -3.3 9.1 69.3 79.0 -54.8  GLY-266   VAL-267  7.6 7.4 -24.9 -8.8 8.5 21.4 363.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ALA-277   GLN-278  5.0 4.4 1.5 -4.1 126.5 132.1 38.6  GLN-278   LYS-279  4.1 3.9 -4.7 -0.9 21.2 24.8 56.8  LYS-279   ILE-280  4.7 4.6 2.4 -3.8 93.9 91.6 -49.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees