Nadp-Dependent Alcohol Dehydrogenase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ILE-194   PHE-195  6.9 6.9 2.1 -8.7 73.8 72.0 49.7
 PHE-195   ALA-196  3.8 3.8 -4.2 -1.9 63.0 64.9 -5.7
 ALA-196   VAL-197  0.6 0.6 -6.0 12.8 126.5 122.4 48.7
 VAL-197   GLY-198  2.6 2.6 -5.9 -49.6 61.4 57.3 571.2
 GLY-198   SER-199  6.0 6.0 7.4 -47.5 54.2 85.0 -251.2
 SER-199   ARG-200  9.6 9.2 25.9 5.1 117.0 146.2 -279.6
 ARG-200   LYS-201  10.4 10.4 -93.2 95.0 98.0 61.3 -267.6
 LYS-201   HIS-202  10.0 10.7 6.9 -35.7 165.3 147.4 327.9
 HIS-202   CYS-203  10.5 9.5 16.0 40.8 113.3 84.5 -72.8
 CYS-203   CYS-204  7.1 6.4 -54.8 14.4 128.9 116.8 -239.0
 CYS-204   ASP-205  5.3 5.9 -18.2 0.0 60.6 53.7 113.8
 ASP-205   ILE-206  7.0 7.2 -0.2 -0.9 42.2 55.2 68.2
 ILE-206   ALA-207  4.5 4.1 -10.2 0.4 112.0 120.7 -97.8
 ALA-207   LEU-208  2.1 2.1 -5.7 4.4 88.7 90.3 -30.9
 LEU-208   GLU-209  5.7 5.8 -5.7 -1.3 28.0 29.8 132.2
 GLU-209   TYR-210  5.4 5.6 0.3 1.9 97.4 95.5 -65.3

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ASP-214   ILE-215  6.8 6.8 6.5 -1.9 94.9 96.9 -27.0
 ILE-215   ILE-216  5.3 5.2 -10.0 16.8 158.8 158.5 122.8
 ILE-216   ASN-217  4.6 4.3 -14.3 -4.0 83.6 84.4 32.0
 ASN-217   TYR-218  7.3 7.2 5.0 -12.9 36.2 21.5 -185.4

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 LYS-234   GLY-235  10.8 10.9 3.8 12.5 58.4 54.6 167.9
 GLY-235   VAL-236  8.4 8.6 -5.1 6.1 155.0 156.7 45.4
 VAL-236   ASP-237  9.2 9.3 2.0 3.2 88.7 86.0 -63.7
 ASP-237   LYS-238  12.5 12.6 1.1 -1.4 71.4 72.0 37.7
 LYS-238   VAL-239  11.6 11.6 2.1 -1.7 80.8 80.2 -13.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees