Endonuclease VIII

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PHE-121   LEU-122  5.6 5.6 -3.2 2.6 53.8 51.0 1.0  LEU-122   GLN-123  5.8 5.8 0.0 6.2 63.3 72.7 3.8  GLN-123   ARG-124  5.3 4.7 0.8 12.3 87.0 100.0 -6.9  ARG-124   VAL-125  1.5 1.0 -7.8 -68.9 32.0 19.9 96.4  VAL-125   GLY-126  0.3 0.8 -29.7 5.8 72.0 55.5 4.4  GLY-126   PRO-127  3.3 3.8 -8.9 -4.1 14.8 19.6 17.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees