Perfringolysin O
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ARG-49
ASN-50
18.1
18.3
-22.5
22.8
156.7
163.7
-7.5
ASN-50
GLU-51
18.2
18.2
-6.6
9.0
136.8
133.4
9.3
GLU-51
VAL-52
20.8
20.8
-13.6
19.6
92.7
90.8
-5.3
VAL-52
LEU-53
20.5
20.5
-3.7
-5.0
134.0
136.7
-73.4
LEU-53
ALA-54
17.7
18.0
92.1
-64.4
30.8
37.4
339.0
ALA-54
SER-55
18.3
18.7
6.3
8.4
39.6
36.0
161.5
SER-55
ASN-56
19.1
18.9
4.1
3.9
46.4
49.2
56.2
ASN-56
GLY-57
20.4
21.1
21.0
-28.2
28.2
19.5
-78.6
GLY-57
ASP-58
19.5
19.9
-76.9
-6.9
128.2
130.8
-692.0
ASP-58
LYS-59
21.5
22.8
39.5
-21.5
95.3
48.8
-96.3
LYS-59
ILE-60
23.6
23.5
17.5
31.8
33.2
30.3
424.5
ILE-60
GLU-61
24.7
24.1
-50.4
23.9
119.9
112.4
-219.4
GLU-61
SER-62
26.6
25.8
34.2
27.6
11.1
20.1
823.0
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LEU-85
THR-86
15.6
16.1
12.5
-10.6
27.9
29.1
11.8
THR-86
THR-87
15.6
15.9
0.4
6.5
63.4
62.6
60.5
THR-87
SER-88
12.5
12.8
-7.9
10.1
152.8
154.3
20.4
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-182
GLU-183
2.4
2.4
0.5
-3.1
3.6
9.2
-40.0
GLU-183
SER-184
3.1
3.0
13.6
-8.9
77.8
77.6
11.4
SER-184
MET-185
4.9
5.2
-0.3
4.5
149.7
147.5
57.6
MET-185
VAL-186
6.2
6.4
-2.1
2.5
74.4
76.1
-7.2
VAL-186
TYR-187
8.1
8.3
-8.7
10.9
120.6
121.9
4.7
TYR-187
SER-188
11.8
11.9
0.6
-5.4
79.6
80.5
-31.5
TYR-187
SER-188
11.8
11.9
0.6
-5.4
79.6
80.5
-31.5
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
TYR-187
SER-188
11.8
11.9
0.6
-5.4
79.6
80.5
-31.5
TYR-187
SER-188
11.8
11.9
0.6
-5.4
79.6
80.5
-31.5
SER-188
LYS-189
13.8
13.9
9.3
-2.4
45.2
40.4
87.3
LYS-189
SER-190
14.0
14.1
-9.8
4.7
156.8
154.0
-49.0
SER-190
GLN-191
14.8
14.9
2.2
3.1
99.1
93.5
-9.6
SER-190
GLN-191
14.8
14.9
2.2
3.1
99.1
93.5
-9.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-190
GLN-191
14.8
14.9
2.2
3.1
99.1
93.5
-9.6
SER-190
GLN-191
14.8
14.9
2.2
3.1
99.1
93.5
-9.6
GLN-191
ILE-192
12.7
12.9
-13.8
5.8
78.7
76.9
-5.3
ILE-192
SER-193
9.3
9.4
-9.0
15.1
32.7
32.1
-96.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-194
ALA-195
10.5
10.5
4.2
7.7
94.9
95.1
-11.8
ALA-195
LEU-196
8.0
8.2
0.1
-5.1
72.6
68.1
14.4
LEU-196
ASN-197
5.0
5.2
9.7
-8.9
31.3
34.9
11.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
VAL-198
ASN-199
8.7
8.7
-5.2
-1.3
105.8
107.8
-81.0
ASN-199
ALA-200
12.4
12.4
-9.8
11.2
177.4
176.3
34.6
ALA-200
LYS-201
13.1
13.1
-11.2
-0.2
114.8
114.9
-64.6
LYS-201
VAL-202
15.4
15.5
-3.1
10.7
101.0
94.3
12.6
VAL-202
LEU-203
12.9
13.3
-12.2
12.1
148.9
146.4
19.9
LEU-203
GLU-204
10.7
10.8
-10.1
15.5
142.6
143.1
16.2
GLU-204
ASN-205
13.7
13.5
7.0
-0.6
80.5
81.7
55.0
ASN-205
SER-206
15.6
15.6
-16.1
17.1
99.0
102.3
54.7
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
LYS-220
VAL-221
6.2
6.1
-4.2
-8.4
126.7
129.5
-101.2
VAL-221
MET-222
4.7
4.7
-4.3
-1.4
141.9
146.7
-98.9
MET-222
ILE-223
2.5
2.7
-6.4
10.9
125.4
125.7
45.3
ILE-223
LEU-224
2.1
2.3
11.7
-12.2
42.8
43.7
21.2
LEU-224
ALA-225
1.7
1.4
-5.3
7.1
134.7
134.7
37.5
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
TYR-226
LYS-227
6.0
5.5
0.4
2.3
35.9
34.2
12.4
LYS-227
GLN-228
6.0
5.4
0.3
-11.5
61.8
64.0
-93.0
GLN-228
ILE-229
9.4
8.7
-2.6
6.0
158.4
153.6
29.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ARG-275
THR-276
11.7
11.4
0.5
1.9
57.9
58.6
-32.2
THR-276
ILE-277
9.0
8.8
-0.6
-2.5
165.8
163.6
-33.8
ILE-277
TYR-278
9.0
8.7
-1.5
3.6
115.9
119.8
-1.8
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
VAL-279
LYS-280
5.8
5.6
-4.8
4.6
59.7
57.3
-32.8
LYS-280
LEU-281
4.6
4.5
3.9
-6.5
148.9
147.9
55.0
LEU-281
GLU-282
2.4
2.1
-18.7
19.0
59.5
56.3
71.1
GLU-282
THR-283
3.2
3.2
17.2
-13.7
15.3
21.3
108.5
GLU-282
THR-283
3.2
3.2
17.2
-13.7
15.3
21.3
108.5
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
GLU-282
THR-283
3.2
3.2
17.2
-13.7
15.3
21.3
108.5
GLU-282
THR-283
3.2
3.2
17.2
-13.7
15.3
21.3
108.5
THR-283
THR-284
2.7
2.5
3.2
-6.4
62.3
61.8
-40.3
THR-284
SER-285
5.0
4.8
-12.1
14.2
109.7
110.9
-7.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-316
SER-317
3.0
3.3
-2.5
6.0
104.8
109.2
57.5
SER-317
PHE-318
6.1
6.0
-17.6
13.8
173.1
170.7
-58.6
PHE-318
THR-319
6.8
6.7
4.9
-3.1
62.4
59.6
39.9
THR-319
ALA-320
9.0
8.5
9.1
-15.5
17.8
21.9
-76.0
ALA-320
VAL-321
10.2
9.9
0.9
-4.1
61.6
63.8
44.1
VAL-321
VAL-322
12.2
11.8
-11.5
7.2
158.8
160.3
-67.5
VAL-322
LEU-323
13.1
12.9
-11.6
20.3
124.8
123.6
72.9
LEU-323
GLY-324
13.3
13.2
-27.3
-36.7
157.9
154.9
-773.0
GLY-324
GLY-325
14.3
15.4
175.9
-10.5
77.4
86.5
607.9
GLY-325
ASP-326
16.7
16.5
50.0
-160.7
124.3
83.0
455.2
ASP-326
ALA-327
19.6
20.0
-21.1
-8.1
3.8
33.9
403.1
ALA-327
GLN-328
18.9
20.8
-68.0
9.5
74.4
70.2
19.3
GLN-328
GLU-329
21.5
20.5
-153.1
-34.0
65.1
29.1
-1937.1
GLU-329
HIS-330
20.2
22.8
178.2
-174.6
173.8
141.1
25.1
HIS-330
ASN-331
18.8
20.8
46.7
-45.6
126.3
72.0
121.4
ASN-331
LYS-332
18.1
17.2
-161.2
55.6
57.5
48.8
913.8
LYS-332
VAL-333
15.5
15.3
-6.5
-9.4
43.5
39.9
144.3
VAL-333
VAL-334
14.2
14.1
1.6
0.9
126.8
126.6
-11.5
VAL-334
THR-335
12.3
12.1
1.5
-1.3
134.4
134.8
6.3
THR-335
LYS-336
11.4
11.4
-6.2
12.8
62.5
62.9
-63.0
LYS-336
ASP-337
9.4
9.5
-1.7
4.1
100.6
105.4
58.1
ASP-337
PHE-338
11.6
11.7
-1.2
-2.4
10.3
12.7
35.4
ASP-337
PHE-338
11.6
11.7
-1.2
-2.4
10.3
12.7
35.4
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ASP-337
PHE-338
11.6
11.7
-1.2
-2.4
10.3
12.7
35.4
ASP-337
PHE-338
11.6
11.7
-1.2
-2.4
10.3
12.7
35.4
PHE-338
ASP-339
10.4
10.5
-8.7
9.2
54.9
56.4
-2.2
ASP-339
GLU-340
14.1
14.2
-6.2
4.3
61.2
59.2
10.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
ILE-341
ARG-342
11.5
11.4
9.1
-7.0
45.3
44.9
19.8
ARG-342
LYS-343
12.9
12.9
-2.8
2.5
120.5
121.9
15.7
LYS-343
VAL-344
15.9
16.0
-5.6
15.0
136.6
135.7
92.9
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
HIS-376
ASN-377
15.3
15.7
-4.3
3.6
154.5
152.6
6.0
ASN-377
LYS-378
15.7
16.1
-1.5
2.3
115.4
118.0
-6.9
LYS-378
THR-379
18.0
18.3
-7.2
13.2
153.2
150.2
91.6
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees