Manganese-Dependent Inorganic Pyrophosphatase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-186   ALA-187  4.6 5.0 -12.6 2.6 154.1 150.7 -7.7  ALA-187   GLY-188  4.5 4.8 19.1 27.8 96.6 95.0 -7.2  GLY-188   ALA-189  4.0 3.6 -57.5 8.4 69.5 111.8 4.8  ALA-189   ASP-190  1.0 1.2 -37.2 139.4 129.0 125.3 80.1  ASP-190   LEU-191  1.4 2.5 -15.7 61.1 112.6 91.6 24.1  LEU-191   SER-192  4.8 5.4 -20.7 6.9 102.3 121.9 15.4  SER-192   LYS-193  8.0 7.9 -17.4 -2.4 170.5 162.0 -23.8  LYS-193   LYS-194  6.6 6.6 22.3 -20.6 82.6 90.2 -3.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees