Ubiquitin-Activating Enzyme E1 1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ALA-913   SER-914  1.4 0.9 -0.7 0.4 116.2 117.7 16.4
 SER-914   PRO-915  2.9 3.1 1.8 4.6 111.1 109.4 -5.7
 PRO-915   LYS-916  6.4 6.8 -7.4 10.2 134.0 129.9 36.1

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 TYR-924   ASP-925  10.9 10.6 -2.1 14.9 121.4 121.5 43.3
 ASP-925   LYS-926  7.4 7.2 -6.9 11.0 80.5 78.0 -19.7
 LYS-926   ILE-927  8.6 8.9 -11.1 10.5 68.7 65.1 -4.0
 ILE-927   TRP-928  6.1 6.7 -6.0 -0.2 156.3 155.4 -39.5
 ILE-927   TRP-928  6.1 6.7 -6.0 -0.2 156.3 155.4 -39.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 ILE-927   TRP-928  6.1 6.7 -6.0 -0.2 156.3 155.4 -39.5
 ILE-927   TRP-928  6.1 6.7 -6.0 -0.2 156.3 155.4 -39.5
 TRP-928   ASP-929  4.0 4.3 11.1 -1.2 44.2 47.2 44.0
 ASP-929   ARG-930  6.8 6.9 2.2 2.0 58.6 54.6 112.5

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees

Residue
i		 Residue
i+1		 Distance of hinge axis to residue i in conformer 1
(A) 		Distance of hinge axis to residue i in conformer 2
(A) 		Change in psi (i)
(deg) 		Change in phi (i+1)
(deg) 		Angle of psi(i) axis to hinge axis conformer 1
(deg) 		Angle of psi(i) axis to hinge axis conformer 2
(deg) 		Percentage Progress
 VAL-989   THR-990  27.5 27.5 20.2 -15.6 79.5 81.8 32.2
 THR-990   LYS-991  26.8 27.0 87.1 -92.9 138.9 137.6 -31.1
 LYS-991   LYS-992  29.2 30.2 7.0 18.7 141.1 140.3 -160.9
 LYS-992   ASP-993  27.0 28.1 9.0 -9.6 69.1 70.6 16.7
 ASP-993   ILE-994  23.5 24.3 -104.4 169.2 51.0 40.0 -172.2
 ILE-994   PRO-995  19.8 21.4 -1.2 59.2 72.9 107.5 175.9
 PRO-995   ALA-996  19.2 18.3 -176.3 162.8 65.2 62.0 98.9
 ALA-996   HIS-997  16.7 17.3 -104.8 19.4 101.2 88.9 -40.7
 HIS-997   VAL-998  14.9 14.6 5.6 20.5 34.8 44.8 162.8
 VAL-998   SER-999  13.2 13.2 7.9 -5.4 111.8 109.9 -7.2
 SER-999   THR-1000  10.0 9.6 -30.5 15.4 143.8 137.0 -26.9
 THR-1000   MET-1001  10.1 9.8 -1.5 14.9 119.9 114.3 56.3
 MET-1001   ILE-1002  12.8 12.6 -6.0 0.7 140.3 148.0 -93.0

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees