A Type Vi-A Crispr-Cas Rna-Guided RNA Ribonuclease, Cas13a

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-510   LYS-511  6.1 3.7 -8.4 9.1 114.8 110.3 -2.7  LYS-511   LEU-512  9.0 6.9 -9.8 6.7 53.9 46.1 0.8  LEU-512   LYS-513  10.1 8.4 -17.3 3.0 86.2 75.9 13.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ASP-722   LYS-723  15.3 14.3 -7.4 5.3 24.1 30.0 -12.7  LYS-723   PHE-724  13.8 12.9 -11.1 7.1 96.6 97.6 11.8  PHE-724   LEU-725  10.4 9.5 -4.2 13.7 104.9 105.4 26.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LYS-727   TYR-728  9.8 7.7 -35.5 16.4 53.1 58.5 14.2  TYR-728   GLU-729  9.3 6.0 9.1 -10.4 87.6 96.9 6.1  GLU-729   GLN-730  12.7 9.1 -14.5 8.0 150.6 149.3 -21.6  GLN-730   ASN-731  12.0 9.3 -0.4 54.6 95.1 82.9 21.6  GLN-730   ASN-731  12.0 9.3 -0.4 54.6 95.1 82.9 21.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLN-730   ASN-731  12.0 9.3 -0.4 54.6 95.1 82.9 21.6  GLN-730   ASN-731  12.0 9.3 -0.4 54.6 95.1 82.9 21.6  ASN-731   ASN-732  9.5 6.2 176.7 155.6 30.7 34.3 -119.1  ASN-732   ASN-733  8.4 6.0 56.9 -37.3 91.1 81.6 30.4  ASN-733   ILE-734  6.0 3.6 -76.7 39.4 35.9 80.7 53.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ILE-747   LYS-748  5.9 5.7 14.4 -16.9 60.4 56.7 -20.5  LYS-748   LEU-749  4.6 4.6 -18.0 19.3 99.4 95.9 -11.9  LEU-749   GLY-750  7.0 7.0 -169.6 165.7 153.9 142.9 26.8 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees