Taq DNA Polymerase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 GLY-635   ARG-636  5.8 4.1 -8.6 22.7 87.2 66.1 4.6
 ARG-636   ASP-637  2.9 1.7 -0.4 -56.6 33.7 40.3 81.3
 ASP-637   ILE-638  0.7 1.1 9.6 -16.2 136.1 128.7 2.6

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees


Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 PHE-667   GLY-668  2.1 1.6 8.5 8.0 19.8 29.8 29.4
 GLY-668   VAL-669  3.4 3.6 -14.6 -8.5 103.2 93.9 -3.9
 VAL-669   LEU-670  4.3 5.0 -3.1 -17.4 111.7 93.9 -8.8
 LEU-670   TYR-671  2.7 3.5 -12.7 43.8 170.7 158.7 51.3
 TYR-671   GLY-672  2.1 2.1 -4.0 22.3 109.5 140.0 28.8
 GLY-672   MET-673  4.9 4.5 61.4 -128.6 94.6 79.6 14.2

Downloading Image

Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees