Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-96   GLY-97  22.2 20.9 46.7 168.1 52.5 65.5 1046.6  GLY-97   ASP-98  19.4 20.4 -171.2 3.3 129.4 108.0 -336.5  ASP-98   ALA-99  17.5 18.2 -48.9 50.2 82.0 81.4 -6.6  ALA-99   VAL-100  16.7 17.6 43.5 -36.0 27.1 31.5 36.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  HIS-169   THR-170  13.0 12.8 16.1 18.4 40.8 45.3 124.8  THR-170   ILE-171  14.9 15.0 -20.7 26.0 113.5 129.2 20.0  ILE-171   SER-172  12.9 13.2 0.1 -20.3 49.5 57.8 -48.9  SER-172   ASP-173  14.5 14.3 28.1 -40.4 29.8 31.0 -37.7  ASP-173   ARG-174  14.2 13.8 7.2 -16.0 95.4 90.1 12.0  ARG-174   ARG-175  11.0 10.9 12.6 -43.3 147.1 145.9 110.0  ARG-174   ARG-175  11.0 10.9 12.6 -43.3 147.1 145.9 110.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  ARG-174   ARG-175  11.0 10.9 12.6 -43.3 147.1 145.9 110.0  ARG-174   ARG-175  11.0 10.9 12.6 -43.3 147.1 145.9 110.0  ARG-175   GLY-176  8.7 8.7 4.8 3.0 72.5 83.8 -92.6  GLY-176   VAL-177  6.0 5.7 -4.4 -0.3 85.4 78.9 21.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees