Nad-Dependent Formate Dehydrogenase (E.C.1.2.1.2)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  TYR-144   CYS-145  4.6 4.5 5.7 1.3 17.9 21.1 92.6  CYS-145   ASN-146  4.9 4.9 5.1 -4.5 65.9 67.2 -107.1  ASN-146   SER-147  2.1 2.1 -2.4 -0.6 47.3 49.2 65.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  SER-334   GLY-335  6.9 6.9 0.0 -0.1 104.1 104.6 -99.1  GLY-335   THR-336  4.7 4.7 4.7 -6.9 138.5 136.0 33.9  THR-336   THR-337  4.4 4.4 0.5 1.7 128.3 131.3 45.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees