Replicative DNA Helicase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue
i
Residue
i+1
Distance of hinge axis to residue i in conformer 1
(A)
Distance of hinge axis to residue i in conformer 2
(A)
Change in psi (i)
(deg)
Change in phi (i+1)
(deg)
Angle of psi(i) axis to hinge axis conformer 1
(deg)
Angle of psi(i) axis to hinge axis conformer 2
(deg)
Percentage Progress
 ILE-146   ALA-147  11.0 11.6 2.5 1.4 39.3 54.1 -0.1
 ALA-147   GLU-148  13.3 14.2 -2.6 -4.8 137.7 148.7 -5.3
 GLU-148   SER-149  10.2 12.0 -4.5 -2.4 81.6 83.0 -3.2
 SER-152   THR-153  11.2 6.8 2.9 -1.1 24.5 67.3 -227.3
 THR-153   GLN-154  11.3 4.2 -1.8 8.7 95.6 161.9 5.9
 GLN-154   PHE-155  10.9 5.8 -177.1 -4.8 150.5 125.0 115.1
 PHE-155   TYR-156  12.4 4.4 1.8 -4.3 51.0 47.9 -1.4
 TYR-156   HIS-157  10.7 2.7 6.4 0.2 55.6 71.6 -0.7
 HIS-157   VAL-158  11.9 4.1 -1.4 -2.0 163.4 166.5 -2.8
 VAL-158   LYS-159  12.4 5.5 3.8 0.9 60.6 46.9 5.2
 LYS-159   ASP-160  10.5 5.1 -7.1 7.6 90.4 104.9 -0.7
 ASP-160   VAL-161  7.1 1.7 0.5 -1.8 57.4 59.3 -1.3
 VAL-161   ALA-162  8.3 2.5 -2.9 -1.8 169.2 160.4 -3.8
 ALA-162   GLU-163  8.8 3.6 -4.4 0.6 112.2 130.2 -1.4
 GLU-163   GLU-164  5.3 1.6 -1.0 4.7 102.2 104.8 2.4
 GLU-164   VAL-165  4.5 2.3 0.1 -0.5 37.6 50.4 -0.1

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Graph shows rotational transition at bending residues and can be used to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees