Crispr-Associated Endonuclease Cas9/csn1
(All numbering and residues are taken from first PDB file)
Bending Residue Dihedral Analysis
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
GLU-668
MET-669
5.5
5.1
0.6
-6.6
122.5
101.5
4.8
MET-669
ALA-670
3.6
2.8
-87.0
63.9
25.6
37.7
17.3
ALA-670
ARG-671
2.4
5.9
113.5
-46.3
135.2
131.2
-41.2
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees
Residue
iResidue
i+1Distance of hinge axis to residue i in
(A) Distance of hinge axis to residue i in
(A) Change in
(deg) Change in
(deg) Angle of psi(i) axis to hinge axis
(deg) Angle of psi(i) axis to hinge axis
(deg) Percentage Progress
SER-827
GLU-828
25.2
27.1
-133.4
2.2
25.5
18.8
93.8
ALA-829
GLY-830
18.6
21.6
141.1
-177.7
118.5
76.4
14.2
GLY-830
PHE-831
16.2
18.4
71.0
1.7
90.2
101.2
-54.7
PHE-831
ILE-832
12.5
18.0
-151.2
47.2
115.6
12.8
-115.3
ILE-832
LYS-833
11.5
18.3
-171.4
-3.7
71.0
72.2
20.0
LYS-833
ARG-834
8.2
16.0
-11.7
-22.7
47.3
114.2
10.0
ARG-834
GLN-835
10.1
13.5
13.7
-25.6
146.2
121.5
8.3
GLN-835
LEU-836
8.5
14.1
20.1
-47.5
83.2
161.8
17.2
LEU-836
VAL-837
5.1
13.6
-9.6
-12.1
78.0
79.7
15.4
VAL-837
GLU-838
6.6
11.8
176.9
-23.4
177.7
76.5
-58.8
GLU-838
THR-839
7.3
8.0
-167.5
-52.5
59.4
105.6
-22.9
THR-839
ARG-840
3.9
7.2
25.7
-8.4
89.7
107.6
11.4
ARG-840
GLN-841
3.2
4.9
-164.4
-27.2
53.3
43.0
93.1
GLN-841
ILE-842
6.6
7.4
12.5
7.5
164.8
151.4
-14.3
Graph shows rotational transition at bending residues and can be used
to identify hinge bending residues.
Probably only informative for interdomain rotations greater than 20 degrees