Barnase (G Specific Endonuclease) (E.C.3.1.27.-)

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-36   ALA-37  6.5 7.3 6.5 -10.1 51.4 46.1 0.1  ALA-37   SER-38  6.6 6.7 -174.1 -17.6 102.1 83.4 81.7  SER-38   LYS-39  3.6 3.4 160.3 -12.0 62.8 90.2 92.9  LYS-39   GLY-40  2.1 5.3 23.3 -137.8 18.7 60.4 -68.7  GLY-40   ASN-41  4.0 4.6 -40.7 0.9 105.4 155.9 -18.2  ASN-41   LEU-42  6.5 6.0 89.1 -5.4 78.6 96.7 6.1  LEU-42   ALA-43  9.6 9.7 20.4 3.2 51.1 60.9 7.4  ALA-43   ASP-44  10.4 10.7 -11.0 23.4 70.8 60.9 -7.0  ASP-44   VAL-45  11.1 10.9 -5.8 -13.5 45.6 55.1 9.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees