Intercellular Adhesion Molecule-1

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-369   ARG-370  3.6 3.5 -9.8 17.2 110.6 109.9 2.0  ARG-370   LEU-371  4.7 4.7 6.5 -12.9 140.3 138.3 51.1  LEU-371   ASP-372  6.3 6.3 -11.3 14.9 114.8 113.1 142.1  ASP-372   GLU-373  9.9 9.8 -7.8 4.5 49.7 53.3 8.3  GLU-373   ARG-374  12.9 12.5 0.7 15.2 125.5 127.4 -116.4  ARG-374   ASP-375  14.0 14.1 -17.4 -20.3 46.1 29.0 544.8  ASP-375   CYS-376  10.9 11.6 50.2 -13.3 135.2 114.6 -421.2 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  CYS-392   GLN-393  8.4 8.3 4.9 -4.9 94.4 91.3 -0.1  GLN-393   ALA-394  7.3 7.2 -1.7 -8.9 25.4 25.4 120.7  ALA-394   TRP-395  5.2 5.1 13.3 -15.5 111.5 105.6 28.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-399   PRO-400  8.4 8.4 -7.5 0.3 88.2 86.7 2.8  PRO-400   GLU-401  4.9 5.0 -3.9 4.9 25.8 28.5 -11.6  GLU-401   LEU-402  3.9 3.8 7.8 -8.9 86.6 82.1 -10.3  LEU-402   LYS-403  0.5 0.6 -1.7 -7.8 49.0 43.9 126.4  LYS-403   CYS-404  3.9 3.9 -4.5 18.9 102.7 106.6 27.7 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-429   CYS-430  10.3 10.2 -9.7 7.9 101.9 102.0 -11.3  CYS-430   ARG-431  6.6 6.7 -17.9 15.6 44.0 45.3 42.7  ARG-431   ALA-432  4.6 4.6 -0.8 -1.0 100.5 99.4 48.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-437   GLU-438  5.0 5.0 5.9 -14.6 142.9 142.5 90.5  GLU-438   VAL-439  3.7 3.5 0.1 6.2 73.1 72.8 59.3  VAL-439   THR-440  2.7 2.8 -16.9 23.9 44.8 44.9 -75.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees