Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  2.7 2.7 29.7 0.0 93.4 104.6 -31.6  PRO-87   GLU-88  3.7 3.9 -67.5 20.5 34.9 33.7 167.0  GLU-88   ILE-89  6.6 6.0 6.7 -2.2 98.3 89.4 -66.6 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  VAL-177   ASN-178  10.2 10.4 -19.1 6.3 97.7 99.5 26.5  ASN-178   LEU-179  7.4 7.6 -35.4 22.6 116.2 114.6 -13.9  LEU-179   PRO-180  8.3 8.5 -7.4 9.9 123.4 114.3 -59.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-181   CYS-182  9.4 8.5 40.4 -37.1 33.3 55.0 -37.5  CYS-182   ASP-183  10.3 10.4 -10.9 15.3 135.3 151.4 16.6  ASP-183   VAL-184  10.4 11.1 -22.0 9.6 144.9 137.4 -51.4 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  7.7 7.9 -1.8 -6.1 57.0 65.9 -1.3  PRO-187   ALA-188  6.6 6.3 -18.6 18.5 104.5 101.8 -15.5  ALA-188   VAL-189  4.7 4.1 -16.0 13.7 65.5 49.8 17.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees